In vivo evaluation of the expression kinetics and immunogenicity of lipid nanoparticle mediated messenger RNA and self-amplifying messenger RNA delivery to the retina. KU Leuven
The current project will focus on two key aspects, namely (i) the degree, duration and location of protein expression and (ii) the toxicity (immunogenicity) of the formulations after IVT injection. Therefore, LNP formulations containing reporter mRNA/saRNA expressing green fluorescent protein (GFP) will be IVT injected in mice and confocal scanning laser ophthalmoscopy (cSLO) will be performed to follow GFP expression in function of time. ...