Mapping dynamic transcription factor hierarchies using pooled CRISPR screening with single-cell epigenome readout KU Leuven
Cellular state transitions, such as those occurring during cancer progression, involve transcriptional switches that are controlled by specific combinations of transcription factors. How such dynamic processes are encoded in the genome sequence as “regulatory programs”, and how transcription factors control the epigenome and activate specific enhancers, remains largely unknown. Single-cell transcriptomics provide a unique opportunity to ...