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Comparison of droplet digital PCR and qPCR for the quantification of Shiga toxin-producing Escherichia coli in bovine feces Universiteit Gent
Multiple TaqMan qPCR and droplet digital PCR (ddPCR) diagnostics for pesticide resistance monitoring and management, in the major agricultural pest Tetranychus urticae Universiteit Gent
BACKGROUND Decisions on which pesticide to use in agriculture are expected to become more difficult, as the number of available chemicals is decreasing. For Tetranychus urticae (T. urticae), a major pest for which a number of candidate markers for pesticide resistance are in place, molecular diagnostics could support decision-making for the rational use of acaricides. RESULTS A suite of 12 TaqMan qPCR assays [G314D (GluCl1), G326E, I321T ...
Comparison of droplet digital PCR and qPCR for the quantification of Shiga toxin-producing Escherichia coli in bovine feces Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
Cattle are considered to be the main reservoir for Shiga toxin-producing Escherichia coli (STEC) and are often the direct or indirect source of STEC outbreaks in humans. Accurate measurement of the concentration of shed STEC in cattle feces could be a key answer to questions concerning transmission of STEC, contamination sources and efficiency of treatments at farm level. Infected animals can be identified and the contamination level quantified ...
The Fungal PCR Initiative's evaluation of in-house and commercial Pneumocystis jirovecii qPCR assays: Toward a standard for a diagnostics assay KU Leuven
Quantitative real-time PCR (qPCR) is increasingly used to detect Pneumocystis jirovecii for the diagnosis of Pneumocystis pneumonia (PCP), but there are differences in the nucleic acids targeted, DNA only versus whole nucleic acid (WNA), and also the target genes for amplification. Through the Fungal PCR Initiative, a working group of the International Society for Human and Animal Mycology, a multicenter and monocenter evaluation of PCP qPCR ...
Comparison of droplet digital PCR and qPCR to quantify shigatoxin-producing Escherichia coli in bovine feces Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
Comparison of droplet digital PCR and qPCR to quantify Shiga toxin-producing Escherichia coli in bovine feces. Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
Comparison of droplet digital PCR and qPCR to quantify Shiga toxin-producing Escherichia coli in bovine feces. Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
Optimal detection protocol of Tetracapsuloides bryosalmonae by environmental DNA: A comparison of qPCR and ddPCR approaches Instituut voor Natuur- en Bosonderzoek
Abstract Investigation of environmental DNA (eDNA) is increasingly used to precisely and non-invasively detect and monitor pathogens. Among these, Tetracapsuloides bryosalmonae is a myxozoan endoparasite that causes proliferative kidney disease (PKD) in salmonid fish. Although the detection of T.?bryosalmonae DNA in water samples has been shown to be promising and successful, method comparison and cross-validation are currently lacking. This ...