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Gluconobacter cerevisiae sp. nov., isolated from the brewery environment
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Three strains, LMG 27748T, LMG 27749 and LMG 27882 with identical MALDI-TOF mass spectra
were isolated from samples taken from the brewery environment. Analysis of the 16S rRNA gene
sequence of strain LMG 27748T revealed that the taxon it represents was closely related to type
strains of the species Gluconobacter albidus (100% sequence similarity), Gluconobacter kondonii
(99.9 %), Gluconobacter sphaericus (99.9%) and Gluconobacter kanchanaburiensis (99.5 %).
DNA-DNA hybridization experiments on the type strains of these species revealed moderate DNA
relatedness values (39-65 %). The three strains used D-fructose, D-sorbitol, meso-erythritol,
glycerol, L-sorbose, ethanol (weakly), sucrose and raffinose as a sole carbon source for growth
(weak growth on the latter two carbon sources was obtained for strains LMG 27748T and LMG
27882). The strains were unable to grow on glucose-yeast extract medium at 37 6C. They
produced acid from meso-erythritol and sucrose, but not from raffinose. D-Gluconic acid, 2-keto-Dgluconic
acid and 5-keto-D-gluconic acid were produced from D-glucose, but not 2,5-diketo-Dgluconic
acid. These genotypic and phenotypic characteristics distinguish strains LMG 27748T,
LMG 27749 and LMG 27882 from species of the genus Gluconobacter with validly published
names and, therefore, we propose classifying them formally as representatives of a novel species,
Gluconobacter cerevisiae sp. nov., with LMG 27748T (5DSM 27644T) as the type strain.
were isolated from samples taken from the brewery environment. Analysis of the 16S rRNA gene
sequence of strain LMG 27748T revealed that the taxon it represents was closely related to type
strains of the species Gluconobacter albidus (100% sequence similarity), Gluconobacter kondonii
(99.9 %), Gluconobacter sphaericus (99.9%) and Gluconobacter kanchanaburiensis (99.5 %).
DNA-DNA hybridization experiments on the type strains of these species revealed moderate DNA
relatedness values (39-65 %). The three strains used D-fructose, D-sorbitol, meso-erythritol,
glycerol, L-sorbose, ethanol (weakly), sucrose and raffinose as a sole carbon source for growth
(weak growth on the latter two carbon sources was obtained for strains LMG 27748T and LMG
27882). The strains were unable to grow on glucose-yeast extract medium at 37 6C. They
produced acid from meso-erythritol and sucrose, but not from raffinose. D-Gluconic acid, 2-keto-Dgluconic
acid and 5-keto-D-gluconic acid were produced from D-glucose, but not 2,5-diketo-Dgluconic
acid. These genotypic and phenotypic characteristics distinguish strains LMG 27748T,
LMG 27749 and LMG 27882 from species of the genus Gluconobacter with validly published
names and, therefore, we propose classifying them formally as representatives of a novel species,
Gluconobacter cerevisiae sp. nov., with LMG 27748T (5DSM 27644T) as the type strain.
Tijdschrift: Int J Syst Evol Microbiol
ISSN: 1466-5026
Volume: 64
Pagina's: 1134-1141
Jaar van publicatie:2014
Trefwoorden:Gluconobacter, brewery