In vivo inhibition of epileptiform afterdischarges in rat hippocampus by light-activated chloride channel, stGtACR2 Universiteit Gent
AimsThe blue light-sensitive chloride-conducting opsin, stGtACR2, provides potent optogenetic silencing of neurons. The present study investigated whether activation of stGtACR2 in granule cells of the dentate gyrus (DG) inhibits epileptic afterdischarges in a rat model. MethodsRats were bilaterally injected with 0.9 mu l of AAV2/7-CaMKII alpha-stGtACR2-fusionred in the DG. Three weeks later, afterdischarges were recorded from the DG by placing ...