Expression, binding, and signaling properties of CRF2(a) receptors endogenously expressed in human retinoblastoma Y79 cells: passage-dependent regulation of functional receptors. Vrije Universiteit Brussel
Endogenous expression of the corticotropin-releasing factor type 2a receptor (CRF2(a)) but not CRF2(b) and CRF2(c) was observed in higher passage cultures of Y79 retinoblastoma cells. Functional studies further demonstrated an increase in CRF2(a) mRNA and protein levels with higher passage numbers (bigger than 25 passages). Although the CRF1 receptor was expressed at higher levels than the CRF2(a) receptor, both receptors were easily ...