Publicaties
Gekozen filters:
Gekozen filters:
Point-of-care HPV molecular diagnostics for a test-and-treat model in high-risk HIV populations Universiteit Gent
Next generation diagnostic molecular pathology Universiteit Antwerpen
Tumor evaluation in pathology is more and more based on a combination of traditional histopathology and molecular analysis. Due to the rapid development of new cancer treatments that specifically target aberrant proteins present in tumor cells, treatment decisions are increasingly based on the molecular features of the tumor. Not only the number of patients eligible for targeted precision medicine, but also the number of molecular targets per ...
Diagnostic accuracy of molecular amplification tests for human african trypanosomiasis-systematic review Instituut voor Tropische Geneeskunde
BACKGROUND: A range of molecular amplification techniques have been developed for the diagnosis of Human African Trypanosomiasis (HAT); however, careful evaluation of these tests must precede implementation to ensure their high clinical accuracy. Here, we investigated the diagnostic accuracy of molecular amplification tests for HAT, the quality of articles and reasons for variation in accuracy. METHODOLOGY: Data from studies assessing diagnostic ...
Endorsing good quality assurance practices in molecular pathology: risks and recommendations for diagnostic laboratories and external quality assessment providers KU Leuven
Quality assurance is an indispensable element in a molecular diagnostic laboratory. The ultimate goal is to warrant patient safety. Several risks that can compromise high quality procedures are at stake, from sample collection to the test performed by the laboratory, the reporting of test results to clinicians, and the organization of effective external quality assessment schemes. Quality assurance should therefore be safeguarded at each level ...
Storage of sputum in cetylpyridinium chloride, OMNIgene.SPUTUM, and ethanol is compatible with molecular tuberculosis diagnostic testing Universiteit Antwerpen Instituut voor Tropische Geneeskunde
We compared cetylpyridinium chloride (CPC), ethanol (ETOH), and OMNIgene.SPUTUM (OMNI) for 28-day storage of sputum at ambient temperature before molecular tuberculosis diagnostics. Three sputum samples were collected from each of 133 smear-positive tuberculosis (TB) patients (399 sputum samples). Each patient's sputum was stored with either CPC, ETOH, or OMNI for 28 days at ambient temperature, with subsequent rpoB amplification targeting a ...
Integration of next-generation sequencing in clinical diagnostic molecular pathology laboratories for analysis of solid tumours; an expert opinion on behalf of IQN Path ASBL KU Leuven
The clinical demand for mutation detection within multiple genes from a single tumour sample requires molecular diagnostic laboratories to develop rapid, high-throughput, highly sensitive, accurate and parallel testing within tight budget constraints. To meet this demand, many laboratories employ next-generation sequencing (NGS) based on small amplicons. Building on existing publications and general guidance for the clinical use of NGS and ...
Diagnostic performance of the IdyllaTM respiratory panel for molecular detection of influenza A/B in patients presenting to primary care with influenza-like illness during 3 consecutive influenza seasons Universiteit Antwerpen
Background: Influenza virus (IFV) is often encountered in primary care. Implementation of a rapid diagnostic test for its detection at the point-of-care would enable discrimination from other viral causes of influenza-like-illness (ILI) and might be helpful in individual patient management. In this study, the diagnostic performance of such a point-of-care platform was evaluated. Methods: Respiratory samples (n = 1490) from ILI-patients in ...
Molecular epidemiology and population structure of Mycobacterium tuberculosis complex strains in tuberculosis patients: implications for diagnostic approaches Instituut voor Tropische Geneeskunde
Tuberculosis (TB) is the 2nd most deadly infectious disease, and its implication for health and economy is increasingly recognized in different countries. In this PhD study, we characterized Mycobacterium tuberculosis complex (MTBc) isolates collected from TB patients in Ethiopia and Niger, two countries in East and West Africa that carry a high burden of TB. In addition, we tested the efficiency of current diagnostic practices for ...
Improving the diagnosis of rifampicin-resistant tuberculosis: programmatic aspects, diagnostic challenges, and molecular epidemiology Instituut voor Tropische Geneeskunde
Tuberculosis (TB) remains the leading infectious disease cause of mortality globally. Resistance to rifampicin (i.e. the most powerful anti-TB drug) impedes optimal management of patients and TB control. Indeed, the majority of rifampicin-resistant (RR) TB patients remain undiagnosed and untreated, thus spreading resistant Mycobacterium tuberculosis complex (MTB) strains. In Rwanda the first RR-TB patients were diagnosed in 1989. However, ...