Projects
Single cell analysis of tumor endothelial cell genomic and transcriptomic heterogeneity KU Leuven
Tumor angiogenesis promotes tumor growth, but current anti-angiogenic treatments lack efficacy and suffer resistance, a formidable unmet medical problem with great societal impact. I hypothesize that the limited success of traditional anti-angiogenics relates to the fact that the angiogenesis field has used the wrong endothelial cell (EC) type for therapy development, i.e. normal ECs (NECs) instead of tumor ECs (TECs), which show genomic ...
Study of the antifungal mode of action of plant defensins using single cell analysis KU Leuven
Yearly, invasive fungal infections (IFIs), such as candidemia and invasive aspergillosis, cause 1.7 million deaths worldwide and this number is still increasing, which can be mainly explained by the increase of the at-risk population, being immunocompromised patients, and the increased use of modern medical devices, such as implants and catheters. Mortality rates associated with IFIs are high (up to 50%) and the number of currently used ...
Functional Precision Oncology: Next-generation treatment rationale for glioblastoma Mapping the drug response heterogeneity using single cell protein analysis KU Leuven
Glioblastoma (GBM) is the most aggressive brain cancer in adults. In spite of intensive treatment, median survival is still only 15 months. Reasons that explain failure to develop more efficient therapies include the high invasiveness of the tumor, its complex genetics, and interpatient and intra-tumoral heterogeneity. Understanding and mapping the complexity of the different tumour cell populations and how each of these respond to therapy, ...
Single cell analysis of stochastic gene expression in persisters. KU Leuven
VyCAP single cell isolation platform for high-quality single-cell analysis KU Leuven
Translational research-oriented technology platform for single cell analysis KU Leuven
Single-cell analysis has become a state-of-the-art method used in translational research. However, a major challenge in the single-cell field is to prevent unwanted cellular stress during cell isolation steps as not to perturb transcriptional processes. Current projects and future research of this translational consortium necessitate such single-cell sorting infrastructure for the rapid and gentle isolation of single cells from clinical ...
Mass cytometry by time-of-flight: next-generation flow cytometry for multiplexed single cell analysis KU Leuven
Describing every single cell in the human body is one of the greatest challenges of our era. Single-cell technologies aim at mapping multiple properties of millions of single cells, both in health and disease. Flow cytometry is still the most important tool for single cell, targeted, protein-based analysis. However, standard fluorescence-based cytometry, which commonly measures <20 markers/cell, hampers major progress to map the enormous ...
Innovative mOSTE+ designed digital lab-on-a-chip for manipulation and downstream single cell analysis KU Leuven
Individual cells in a population can substantially differ at the genomic and functional level. Getting further insight into these differences might be a key to answering many of the remaining questions linked to different physiological or pathological processes. Although single cell analysis is rapidly evolving field, the methods are often not amenable to spatio-temporal studies in individual cells in situ and are accompanied by cumbersome ...