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Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis Ghent University
Positive feedback keeps duration of mitosis temporally insulated from upstream cell-cycle events KU Leuven
Cell division is characterized by a sequence of events by which a cell gives rise to two daughter cells. Quan- titative measurements of cell-cycle dynamics in sin- gle cells showed that despite variability in G1-, S-, and G2 phases, duration of mitosis is short and remarkably constant. Surprisingly, there is no corre- lation between cell-cycle length and mitotic duration, suggesting that mitosis is temporally insulated from variability in ...
Effects of tyrosine kinase and phosphatase inhibitors on mitosis progression in synchronized tobacco BY-2 cells University of Antwerp
To test whether reversible tubulin phosphorylation plays any role in the process of plant mitosis the effects of inhibitors of tyrosine kinases, herbimycin A, genistein and tyrphostin AG 18, and of an inhibitor of tyrosine phosphatases, sodium orthovanadate, on microtubule organization and mitosis progression in a synchronized BY-2 culture has been investigated. It was found that treatment with inhibitors of tyrosine kinases of BY-2 cells at the ...
Effect of serine/threonine protein kinases and protein phosphatases inhibitors on mitosis progression in a synchronized tobacco BY-2 culture University of Antwerp
In order to investigate the role of various serine/threonine protein kinases and protein phosphatases in the regulation of mitosis progression in plant cells, the influence of cyclin(olomoucine) and Ca2+/calmodulin-dependent (W7) protein kinases inhibitors, as well as protein kinase C inhibitors (H7 and staurosporine), and a protein phosphatases inhibitor (okadaic acid) on mitosis progression in synchronized tobacco BY-2 cells has been studied. ...
Micronucleation by mitosis inhibitors in developing microspores of Spathiphyllum wallisii Regel Research Institute for Agriculture, Fisheries and Food Ghent University
KEY MESSAGE : We developed an efficient protocol for chromosome scattering in Spathiphyllum microspores. The effects of plant material, developmental age, genotype and antimicrotubular toxin type, exposure and concentration were evaluated. Asymmetric hybridization through microprotoplast-mediated chromosome transfer (MMCT) is a known method for overcoming sexual breeding barriers between distantly related plant species. To obtain ...
The Importance of Kinase–Phosphatase Integration: Lessons from Mitosis KU Leuven
Kinases and phosphatases work antagonistically to control the behaviour of individual substrate molecules. This can be incorrectly extrapolated to imply that they also work antagonistically on the signals or processes that these molecules control. In fact, in many situations kinases and phosphatases work together to positively drive signal responses. We explain how this 'cooperativity' is critical for setting the amplitude, localisation, timing, ...
Micronucleation by mitosis inhibitors in developing Spathiphyllum wallisii Regel microspores for microprotoplast preparation Research Institute for Agriculture, Fisheries and Food
Asymmetric protoplast fusion through microprotoplast mediated chromosome transfer (MMCT) is a known method for overcoming sexual breeding barriers between distantly related plant species. To obtain microprotoplasts, it is necessary to induce mass micronucleation either in somatic or gametic cells. We chose to use pollen mother cells as starting material. We have tested the efficiency for micronuclei induction of 5 mitosis inhibitors, ...