A study of the process of synchronisation and micronucleation in Beta vulgaris and the monitoring of an isolation procedure for micro-nuclei and micro-protoplasts by confocal laser scanning microscopy and flow cytometry Vrije Universiteit Brussel
The process of synchronization and micro-
nuclei induction in a suspension culture of Beta
vulgaris, was induced by the sequential treatment with
the DNA-synthesis inhibitor aphidicolin (30 mM,
24 h) and the spindle-toxin amiprophos-methyl
(32 mM, 24 h). Mitotic arrest of divisions, spreading
of G2-metaphase chromosomes, re-grouping of chromosomes,
formation of a nuclear cell wall around
single and ...
nuclei induction in a suspension culture of Beta
vulgaris, was induced by the sequential treatment with
the DNA-synthesis inhibitor aphidicolin (30 mM,
24 h) and the spindle-toxin amiprophos-methyl
(32 mM, 24 h). Mitotic arrest of divisions, spreading
of G2-metaphase chromosomes, re-grouping of chromosomes,
formation of a nuclear cell wall around
single and ...