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Chromatin structure of two genomic sites for targeted transgene integration in induced pluripotent stem cells and hematopoietic stem cells Vrije Universiteit Brussel

R van Rensburg, Xiaodan Yao, Hengzhe Wang, O Denisenko, Li Zeng, D W Russell, D G Miller, P Gregory, M Holmes, K Bomsztyk, et al.

Achieving transgene integration into preselected genomic sites is currently one of the central tasks in stem cell gene therapy. A strategy to mediate such targeted integration involves site-specific endonucleases. Two genomic sites within the MBS85 and chemokine (C-C motif) receptor 5 (CCR5) genes (AAVS1 and CCR5 zinc-finger nuclease (CCR5-ZFN) sites, respectively) have recently been suggested as potential target regions for integration as ...

Human Wharton's Jelly-Derived Stem Cells Display a Distinct Immunomodulatory and Proregenerative Transcriptional Signature Compared to Bone Marrow-Derived Stem Cells Universiteit Hasselt

Raf DONDERS, Jeroen BOGIE, Stylianos Ravanidis, Pascal GERVOIS, Marjan Vanheusden, Raphaël Marée, Marie Schrynemackers, Hubert J.M. Smeets, Jef Pinxteren, Kristel Gijbels, et al.
Mesenchymal stromal cells (MSCs) are multipotent stem cells with immunosuppressive and trophic support functions. While MSCs from different sources frequently display a similar appearance in culture, they often show differences in their surface marker and gene expression profiles. Although bone marrow is considered the ‘‘gold standard’’ tissue to isolate classical MSCs (BM-MSC), MSC-like cells are currently also derived from more easily ...

In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly-L-lysine-treated titanium-6-aluminium-4-vanadium KU Leuven

Daniela Galli, Laura Benedetti, Matilde Bongio, Valentina Maliardi, Giulia Silvani, Gabriele Ceccarelli, Flavio Ronzoni, Silvio Conte, Francesco Benazzo, Antonio Graziano, et al.
Three-dimensional (3D) titanium-6-aluminium-4-vanadium (Ti6Al4V) is a widely used biomaterial for orthopedic prosthesis and dental implants; thanks to its very high-mechanical strength and resistance to corrosion. Human mesenchymal stem cells (hMSCs) and dental pulp stem cells (hDPSCs) are responsible for bone regeneration following colonization of prosthesis or dental implants. Both hMSCs and hDPSCs have lower ability to colonize this ...

Mesenchymal stem cell secreted platelet derived growth factor exerts a pro-migratory effect on resident Cardiac Atrial appendage Stem Cells Universiteit Hasselt

Severina WINDMOLDERS, Astrid De Boeck, Remco KONINCKX, Annick Daniëls, Olivier De Weyer, Marc Bracke, Marc HENDRIKX, Karen HENSEN, Jean-Luc RUMMENS
Mesenchymal stem cells (MSCs) modulate cardiac healing after myocardial injury through the release of paracrine factors, but the exact mechanisms are still unknown. One possible mechanism is through mobilization of endogenous cardiac stemcells (CSCs). This study aimed to test the pro-migratory effect ofMSC conditionedmedium (MSC-CM) on endogenous CSCs from human cardiac tissue. By using a three-dimensional collagen assay, we found that MSC-CM ...

Embryonic Stem Cell-Derived Mesenchymal Stem Cells (MSCs) Have a Superior Neuroprotective Capacity Over Fetal MSCs in the Hypoxic-Ischemic Mouse Brain KU Leuven

Kate E Hawkins, Michelangelo Corcelli, Kate Dowding, Anna M Ranzoni, Filipa Vlahova, Kwan-Leong Hau, Avina Hunjan, Donald Peebles, Pierre Gressens, Henrik Hagberg, et al.
Human mesenchymal stem cells (MSCs) have huge potential for regenerative medicine. In particular, the use of pluripotent stem cell-derived mesenchymal stem cells (PSC-MSCs) overcomes the hurdle of replicative senescence associated with the in vitro expansion of primary cells and has increased therapeutic benefits in comparison to the use of various adult sources of MSCs in a wide range of animal disease models. On the other hand, fetal MSCs ...

Spermatogonial stem cells. An update on spermatogonial stem cell banking and transplantation Vrije Universiteit Brussel

Ellen Goossens, Dorien Van Saen, Mieke Geens, Liang Ning, Herman Tournaye
Like every other adult stem cell in the human body, spermatogonial stem cells (SSCs) have the capacity to either renew themselves or to start differentiation, i.e. spermatogenesis. Due to these properties, several options for preservation and re-establishment of the spermatogenic process exist.
Prevention of sterility has become an important issue in reproductive medicine. For adult men, sterility after cancer treatment can be circumvented ...

Co-transplantation of mesenchymal stem cells improves spermatogonial stem cell transplantation efficiency in mice Vrije Universiteit Brussel

Prashant Kadam, Elissavet Ntemou, Yoni Baert, Sven Van Laere, Dorien Van Saen, Ellen Goossens
Background: Spermatogonial stem cell transplantation (SSCT) could become a fertility restoration tool for childhood cancer survivors. However, since in mice, the colonization efficiency of transplanted spermatogonial stem cells (SSCs) is only 12%, the efficiency of the procedure needs to be improved before clinical implementation is possible. Co-transplantation of mesenchymal stem cells (MSCs) might increase colonization efficiency of SSCs by ...