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The quaternary structure of human tyrosine hydroxylase: effects of dystonia-associated missense variants on oligomeric state and enzyme activity Vlaams Instituut voor Biotechnologie Vrije Universiteit Brussel
Top-down mass spectrometry of intact membrane protein complexes reveals oligomeric state and sequence information in a single experiment Universiteit Antwerpen
Here we study the intact stoichiometry and top-down fragmentation behavior of three integral membrane proteins which were natively reconstituted into detergent micelles: the mechano-sensitive ion channel of large conductance (MscL), the Kirbac potassium channel and the p7 viroporin from the hepatitis C virus. By releasing the proteins under nondenaturing conditions inside the mass spectrometer, we obtained their oligomeric sizes. Increasing the ...
Small-angle X-ray scattering studies of the oligomeric state and quaternary structure of the trifunctional proline utilization A (PutA) flavoprotein from Escherichia coli Vrije Universiteit Brussel
The trifunctional flavoprotein proline utilization A (PutA) links metabolism and gene regulation in Gram-negative bacteria by catalyzing the two-step oxidation of proline to glutamate and repressing transcription of the proline utilization regulon. Small-angle x-ray scattering (SAXS) and domain deletion analysis were used to obtain solution structural information for the 1320-residue PutA from Escherichia coli. Shape reconstructions show that ...
The Cryo-EM structure of AAV2 Rep68 in complex with ssDNA reveals a malleable AAA+ machine that can switch between oligomeric states KU Leuven
The adeno-associated virus (AAV) non-structural Rep proteins catalyze all the DNA transactions required for virus viability including, DNA replication, transcription regulation, genome packaging, and during the latent phase, site-specific integration. Rep proteins contain two multifunctional domains: an Origin Binding Domain (OBD) and a SF3 helicase domain (HD). Studies have shown that Rep proteins have a dynamic oligomeric behavior where the ...
A Generic Mechanism of Emergence of Amyloid Protofilaments from Disordered Oligomeric Aggregates KU Leuven
The presence of oligomeric aggregates, which is often observed during the process of amyloid formation, has recently attracted much attention because it has been associated with a range of neurodegenerative conditions including Alzheimer's and Parkinson's diseases. We provide a description of a sequence-indepedent mechanism by which polypeptide chains aggregate by forming metastable oligomeric intermediate states prior to converting into ...
The first observation of the E,Z configuration of Ar-X-N=S=N-X-Ar (X = S, Se) chains in the crystalline state Universiteit Antwerpen
New oligomeric analogues of poly(sulfur nitride), i.e. 3-ClC6R4XN=S=NXC6R4Cl-3 (58; R = H, F and X = S, Se), were synthesized and structurally characterized in the solid state by single-crystal XRD, in solution by variable-temperature NMR spectroscopy and in the gas phase with DFT/B3LYP calculations. In the crystal, compounds 57 display the well-known Z,Z configuration, whereas 8 (R = F, X = Se) is the first compound to display the E,Z ...
Native ion mobility-mass spectrometry reveals the formation of beta-barrel shaped amyloid-beta hexamers in a membrane-mimicking environment Universiteit Antwerpen
The mechanisms behind the Amyloid-beta (A beta) peptide neurotoxicity in Alzheimer's disease are intensely studied and under debate. One suggested mechanism is that the peptides assemble in biological membranes to form beta-barrel shaped oligomeric pores that induce cell leakage. Direct detection of such putative assemblies and their exact oligomeric states is however complicated by a high level of heterogeneity. The theory consequently remains ...
Biochemical and biophysical characterization of human small heat shock proteins KU Leuven
Small heat shock proteins play an important role in maintaining the quality of all proteins in a cell, both under normal and stress conditions and are part of a large network called the protein quality control network. sHSPs can be considered as a first line of defense against unfolding protein species under stress conditions, this is because of their ATP-independent mode of action. These chaperones can bind partially unfolded proteins and trap ...
Investigation of inorganic material growth processes KU Leuven
In situ investigation of inorganic material growth processes. Synthetic nanoporous silicate based materials contribute substantially to the development of a sustainable society. Silica gels, ordered mesoporous silica and zeolites are essential to catalysis and molecular separation in chemical production and environmental protection, conversion and storage of energy, detergent builders and daily, new applications are being discovered. The ...