Publicaties
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Camelid reporter gene imaging: A generic method for in vivo cell tracking Vrije Universiteit Brussel
BACKGROUND:
To combine the sensitivity of bioluminescent imaging (BLI) with the 3D and quantitative properties of pinhole single-photon emission computed tomography (SPECT)/micro-computed tomography (CT) (phSPECT/micro-CT), we generated stable cell lines that express a yellow-fluorescent protein (YFP) and Gaussia luciferase (GLuc) fusion protein (YFP/GLuc). For in vivo phSPECT detection of this YFP/GLuc protein, a nanobody, targeted ...
To combine the sensitivity of bioluminescent imaging (BLI) with the 3D and quantitative properties of pinhole single-photon emission computed tomography (SPECT)/micro-computed tomography (CT) (phSPECT/micro-CT), we generated stable cell lines that express a yellow-fluorescent protein (YFP) and Gaussia luciferase (GLuc) fusion protein (YFP/GLuc). For in vivo phSPECT detection of this YFP/GLuc protein, a nanobody, targeted ...
Nanobody-based targeted radionuclide therapy for cancer treatment Vrije Universiteit Brussel
Mapping the Binding Interface between an HIV-1 Inhibiting Intrabody and the Viral Protein Rev Vrije Universiteit Brussel KU Leuven
HIV-1 Rev is the key protein in the nucleocytoplasmic export and expression of the late viral mRNAs. An important aspect for its function is its ability to multimerize on these mRNAs. We have recently identified a llama single-domain antibody (Nb190) as the first inhibitor targeting the Rev multimerization function in cells. This nanobody is a potent intracellular antibody that efficiently inhibits HIV-1 viral production. In order to gain ...
Downsizing the downsized Vrije Universiteit Brussel
Antibodies represent an important therapeutic drug family thanks to their high affinity and specific binding capabilities. However, they suffer from several drawbacks, such as a high production cost and limited cellular access, due to their overall molecular architecture and high molecular weight. For these reasons, downsized functional antibody fragments were developed, among which the popular single variable heavy domains named nanobodies ...
Nanobody-based pannexin1 channel inhibitors reduce inflammation in acute liver injury Vrije Universiteit Brussel
Background: The opening of pannexin1 channels is considered as a key event in inflammation. Pannexin1 channel-mediated release of adenosine triphosphate triggers inflammasome signaling and activation of immune cells. By doing so, pannexin1 channels play an important role in several inflammatory diseases. Although pannexin1 channel inhibition could represent a novel clinical strategy for treatment of inflammatory disorders, therapeutic pannexin1 ...
Generation and characterization of nanobodies targeting PSMA for molecular imaging of prostate cancer Vrije Universiteit Brussel
Nanobodies show attractive characteristics for tumor targeting in cancer diagnosis and therapy. A radiolabeled nanobody binding the prostate-specific membrane antigen (PSMA) could offer a noninvasive strategy to select prostate cancer patients eligible for PSMA-targeted therapies. We here describe the generation, production and in vivo evaluation of anti-PSMA nanobodies. Nanobodies were derived from heavy-chain-only antibodies, raised in ...
A novel nanobody-based bio-assay using functional complementation of a split nanoluciferase to monitor Mu- opioid receptor activation Universiteit Gent
Generation and characterization of small single domain antibodies inhibiting human TNF receptor 1 Vrije Universiteit Brussel Universiteit Gent
The cytokine TNF is a well-known drug target for several inflammatory diseases such as Crohn's disease (CD). Despite the great success of TNF blockers, therapy could be improved because of high costs and side-effects. Selective inhibition of TNFR1 signaling holds the potential to greatly reduce the pro-inflammatory activity of TNF, thereby preserving the advantageous immunomodulatory signals mediated by TNFR2. We generated a selective human ...
Structure of an early native-like intermediate of beta2-microglobulin amyloidogenesis Vrije Universiteit Brussel
To investigate early intermediates of ?2-microglobulin (b2m) amyloidogenesis, we solved the structure of b2m containing the amyloidogenic Pro32Gly mutation by X-ray crystallography. One nanobody (Nb24) that efficiently blocks fibril elongation was used as a chaperone to co-crystallize the Pro32Gly b2m monomer under physiological conditions. The complex of P32G b2m with Nb24 reveals a trans peptide bond at position 32 of this amyloidogenic ...