Modulation of protein properties in living cells using nanobodies. Vrije Universiteit Brussel
Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and spectral properties of the green fluorescent protein (GFP). One nanobody could reversibly reduce GFP fluorescene by a factor of 5, whereas its displacement by a second nanobody caused an increase by a factor of ...