Publicaties
Should isoniazid and clofazimine be used to treat multidrug-resistant tuberculosis? [reply] Instituut voor Tropische Geneeskunde
Optimization of the clofazimine structure leads to a highly water-soluble C3-aminopyridinyl riminophenazine endowed with improved anti-Wnt and anti-cancer activity in vitro and in vivo KU Leuven
Multicentre study to establish interpretive criteria for clofazimine drug susceptibility testing Instituut voor Tropische Geneeskunde Universiteit Antwerpen
OBJECTIVE: To conduct a multicentre study to establish the critical concentration (CC) for clofazimine (CFZ) for drug susceptibility testing (DST) of Mycobacterium tuberculosis on the MGIT (TM) 960 (TM) system using the distribution of minimum inhibitory concentrations (MIC) and genotypic analyses of Rv0678 mutations.
DESIGN: In phase I of the study, the MIC distribution of laboratory strains (H37Rv and in vitro-selected Rv0678 ...
Unexpected high prevalence of resistance-associated Rv0678 variants in MDR-TB patients without documented prior use of clofazimine or bedaquiline Instituut voor Tropische Geneeskunde
OBJECTIVES: Resistance-associated variants (RAVs) in Rv0678, a regulator of the MmpS5-MmpL5 efflux pump, have been shown to lead to increased MICs of bedaquiline (2- to 8- fold) and clofazimine (2- to 4-fold). The prevalence of these Rv0678 RAVs in clinical isolates and their impact on treatment outcomes are important factors to take into account in bedaquiline treatment guidelines.
METHODS: Baseline isolates from two bedaquiline ...
Bedaquiline- and clofazimine- selected Mycobacterium tuberculosis mutants: further insights on resistance driven largely by Rv0678 Instituut voor Tropische Geneeskunde
Drug-resistant tuberculosis is a serious global health threat. Bedaquiline (BDQ) is a relatively new core drug, targeting the respiratory chain in Mycobacterium tuberculosis (Mtb). While mutations in the BDQ target gene, atpE, are rare in clinical isolates, mutations in the Rv0678 gene, a transcriptional repressor regulating the efflux pump MmpS5-MmpL5, are increasingly observed, and have been linked to worse treatment outcomes. Nevertheless, ...
Sterilizing activities of novel combinations lacking first- and second-line drugs in a murine model of tuberculosis Universiteit Antwerpen
Short, highly effective and inexpensive standardized treatment of multidrug-resistant tuberculosis Instituut voor Tropische Geneeskunde
Injectables' key role in rifampicin-resistant tuberculosis shorter treatment regimen outcomes Instituut voor Tropische Geneeskunde
Background
Since a meta-analysis showed little or no effect of second-line injectables on treatment success, and using injectables may induce ototoxicity, injectable-free rifampicin-resistant tuberculosis (RR-TB) treatment regimens are recommended. However, acquired resistance preventing activity was overlooked. No previous study assessed the effect of shortening the duration of kanamycin administration to 2 months during the ...
Validation of bedaquiline phenotypic drug susceptibility testing methods and breakpoints: a multilaboratory, multicountry study Instituut voor Tropische Geneeskunde
Drug-resistant tuberculosis persists as a major public health concern. Alongside efficacious treatments, validated and standardized drug susceptibility testing (DST) is required to improve patient care. This multicountry, multilaboratory external quality assessment (EQA) study aimed to validate the sensitivity, specificity, and reproducibility of provisional bedaquiline MIC breakpoints and World Health Organization interim critical ...