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Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells Universiteit Gent

Laurens Pauwels, Kris Morreel, Emilie De Witte, Freya Lammertyn, Marc Van Montagu, Wout Boerjan, Dirk Inzé, Alain Goossens
Jasmonates (JAs) are plant-specific signaling molecules that steer a diverse set of physiological and developmental processes. Pathogen attack and wounding inflicted by herbivores induce the biosynthesis of these hormones, triggering defense responses both locally and systemically. We report on alterations in the transcriptome of a fast-dividing cell culture of the model plant Arabidopsis thaliana after exogenous application of methyl JA (MeJA). ...

Single-cell PCR analysis of murine embryonic stem cells cultured on different substrates highlights heterogeneous expression of stem cell markers KU Leuven

Paolo De Coppi
BACKGROUND INFORMATION: In the last few years, recent evidence has revealed that inside an apparently homogeneous cell population there indeed appears to be heterogeneity. This is particularly true for embryonic stem (ES) cells where markers of pluripotency are dynamically expressed within the single cells. In this work, we have designed and tested a new set of primers for multiplex PCR detection of pluripotency markers expression, and have ...

alpha-Skeletal muscle actin nemaline myopathy mutants cause cell death in cultured muscle cells. Universiteit Gent

Drieke Vandamme, Ellen Lambert, Davy Waterschoot, C. Cognard, Joël Vandekerckhove, Christophe Ampe, B. Constantin, Heidi Rommelaere
Nemaline myopathy is a neuromuscular disorder, characterized by muscle weakness and hypotonia and is, in 20% of the cases, caused by mutations in the gene encoding alpha-skeletal muscle actin, ACTA1. It is a heterogeneous disease with various clinical phenotypes and severities. In patients the ultrastructure of muscle cells is often disturbed by nemaline rods and it is thought this is the cause for muscle weakness. To search for possible defects ...

Differentiating neural crest stem cells induce proliferation of cultured rodent islet beta cells Vrije Universiteit Brussel

Gael Grouwels, Svetlana Vasylovska, Johan Olerud, Gunter Leuckx, Anongnad Ngamjaeiyawat, Yixing Yuchi, Leif Jansson, Mark Van De Casteele, Elena Kozlova, Henry Heimberg, et al.
AIMS/HYPOTHESIS:

Efficient stimulation of cycling activity in cultured beta cells would allow the design of new strategies for cell therapy in diabetes. Neural crest stem cells (NCSCs) play a role in beta cell development and maturation and increase the beta cell number in co-transplants. The mechanism behind NCSC-induced beta cell proliferation and the functional capacity of the new beta cells is not known.

METHODS:
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Immunocytochemical characterization of ex vivo cultured conjunctival explants ; marker validation for the identification of squamous epithelial cells and goblet cells Universiteit Antwerpen

Sara Van Acker, Bert Van den Bogerd, Michel Haagdorens, Carina Koppen, Isabel Pintelon
Tissue-engineered products are at the cutting edge of innovation considering their potential to functionally and structurally repair various tissue defects when the body's own regenerative capacity is exhausted. At the ocular surface, the wound healing response to extensive conjunctival damage results in tissue repair with structural alterations or permanent scar formation rather than regeneration of the physiological conjunctiva. Conjunctival ...

Allogeneic Human Mesenchymal Stem Cells Restore Epithelial Protein Permeability in Cultured Human Alveolar Type II Cells by Secretion of Angiopoietin-1 KU Leuven

Arne Neyrinck
Acute lung injury is characterized by injury to the lung epithelium that leads to impaired resolution of pulmonary edema and also facilitates accumulation of protein-rich edema fluid and inflammatory cells in the distal airspaces of the lung. Recent in vivo and in vitro studies suggest that mesenchymal stem cells (MSC) may have therapeutic value for the treatment of acute lung injury. Here we tested the ability of human allogeneic mesenchymal ...

Evaluation of the Suitability of Biocompatible Carriers as Artificial Transplants Using Cultured Porcine Corneal Endothelial Cells Vrije Universiteit Brussel

Daniele Spinozzi, Alina Miron, Marieke Bruinsma, Isabel Dapena, Itay Lavy, Perry S. Binder, Mehrdad Rafat, Silke Oellerich, Gerrit R J Melles

Purpose/Aim: Evaluating the suitability of bioengineered collagen sheets and human anterior lens capsules (HALCs) as carriers for cultivated porcine corneal endothelial cells (pCECs) and in vitro assessment of the cell-carrier sheets as tissue-engineered grafts for Descemet membrane endothelial keratoplasty (DMEK). Materials and Methods: pCECs were isolated, cultured up to P2 and seeded onto LinkCell™ bioengineered matrices of 20 µm ...

In Vitro Evaluation and Transplantation of Human Corneal Endothelial Cells Cultured on Biocompatible Carriers Vrije Universiteit Brussel Universiteit Antwerpen

Daniele Spinozzi, Alina Miron, Jessica T Lie, Mehrdad Rafat, Neil Lagali, Gerrit R J Melles, Sorcha Ní Dhubhghaill, Isabel Dapena, Silke Oellerich

Corneal transplantation is currently the only effective treatment option for dysfunctional corneal endothelial cells (CEC). In this study, we test in vitro the surgical potential of cultivated human corneal endothelial cells (hCEC) on human anterior lens capsule (HALC), LinkCell™ bioengineered collagen sheets of 20-µm thickness (LK20), and denuded Descemet membrane (dDM) as tissue-engineered grafts for Descemet membrane (DM) endothelial ...

Cultured human peripheral blood mononuclear cells alter their gene expression when challenged with endocrine-disrupting chemicals Universiteit Hasselt Universiteit Antwerpen

B. Wens, Patrick DE BOEVER, M. Verbeke, K. Hollanders, G. Schoeters
Endocrine disrupting chemicals (EDCs) have the potential to interfere with the hormonal system and may negatively influence human health. Microarray analysis was used in this study to investigate differential gene expression in human peripheral blood cells (PBMCs) after in vitro exposure to EDCs. PBMCs, isolated from blood samples of four male and four female healthy individuals, were exposed in vitro for 18 h to either a dioxin-like ...