Publicaties

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Discovery and 3D imaging of a novel ΔNp63-expressing basal cell type in human pancreatic ducts with implications in disease Vrije Universiteit Brussel

Sandrina Martens, Katarina Coolens, Mathias Van Bulck, Yves Heremans, Gunter Leuckx, Henry Heimberg, Luc Bouwens, Patrick Jacquemin, Diedert Luc De Paep

OBJECTIVE: The aggressive basal-like molecular subtype of pancreatic ductal adenocarcinoma (PDAC) harbours a ΔNp63 (p40) gene expression signature reminiscent of a basal cell type. Distinct from other epithelia with basal tumours, ΔNp63+ basal cells reportedly do not exist in the normal pancreas.

DESIGN: We evaluated ΔNp63 expression in human pancreas, chronic pancreatitis (CP) and PDAC. We further studied in depth the non-cancerous ...

Undetectable viral RNA in follicular fluid, cumulus cells, and endometrial tissue samples in SARS-CoV-2-positive women Vrije Universiteit Brussel

Liese Boudry, Wafaa Essahib, Ileana Mateizel, Hilde Van De Velde, Deborah De Geyter, Denis Pierard, Wim Waelput, Valerie Uvin, Herman Tournaye, Michel De Vos, et al.

OBJECTIVE: To study the presence of viral RNA in the follicular fluid, cumulus cells, and endometrial tissue samples in SARS-CoV-2-positive women undergoing assisted reproductive technology (ART).

DESIGN: Prospective, single-center, observational study.

SETTING: Tertiary hospital.

PATIENT(S): A total of 16 patients undergoing transvaginal oocyte retrieval who had a positive SARS-CoV-2 RNA test <48 hours before the ...

Efficacy of a Flat Low Dose of Nivolumab in Advanced Cancer Vrije Universiteit Brussel

Sofie Joris, Christel Fontaine, Lore Decoster, Jacques Vanderauwera, Kris Thielemans, Wim Waelput, Jacques De Grève

BACKGROUND/AIM: Immunotherapy with PD-1/PDL1 blocking monoclonal antibodies has improved survival compared to the standard-of-care chemotherapy for several malignancies at different stages of these malignancies. Due to several reasons, many cancer patients in medical need have no access to these drugs. In this study, we aimed to investigate whether a low dose of nivolumab could also lead to a therapeutic response.

PATIENTS AND METHODS: ...

Assessing Tumor-Infiltrating Lymphocytes in Breast Cancer Vrije Universiteit Brussel

Hanne Locy, Stefaan Verhulst, Wilfried Cools, Wim Waelput, Stefanie Brock, Louise Cras, Ann Schiettecatte, Jan Jonckheere, Leo A van Grunsven, Marian Vanhoeij, et al.

Scoring of tumor-infiltrating lymphocytes (TILs) in breast cancer specimens has gained increasing attention, as TILs have prognostic and predictive value in HER2+ and triple-negative breast cancer. We evaluated the intra- and interrater variability when scoring TILs by visual inspection of hematoxylin and eosin-stained tissue sections. We further addressed whether immunohistochemical staining of these sections for immune cell surface markers ...

Comprehensive genome-wide analysis of routine non-invasive test data allows cancer prediction Vrije Universiteit Brussel KU Leuven

Liesbeth Lenaerts, Nathalie Brison, Charlotte Maggen, Leen Vancoillie, Huiwen Che, Peter Vandenberghe, Daan Dierickx, Lucienne Michaux, Barbara Dewaele, Patrick Neven, et al.

Background: Implausible false positive results in non-invasive prenatal testing (NIPT) have been occasionally associated with the detection of occult maternal malignancies. Hence, there is a need for approaches allowing accurate prediction of whether the NIPT result is pointing to an underlying malignancy, as well as for organized programs ensuring efficient downstream clinical management of these cases.

Methods: Using a data set of ...

Overcoming the Challenges of High Quality RNA Extraction from Core Needle Biopsy Vrije Universiteit Brussel

Hanne Locy, Rohann J.M. Correa, Dorien Autaers, Ann Schiettecatte, Jan Jonckheere, Wim Waelput, Louise Cras, Stefanie Brock, Stefaan Verhulst, Keith Kwan, et al.
The use of gene expression profiling (GEP) in cancer management is rising, as GEP can be used for disease classification and diagnosis, tailoring treatment to underlying genetic determinants of pharmacological response, monitoring of therapy response, and prognosis. However, the reliability of GEP heavily depends on the input of RNA in sufficient quantity and quality. This highlights the need for standard procedures to ensure best practices for ...

Evaluation of an online training tool for scoring programmed cell death ligand-1 (PD-L1) diagnostic tests for lung cancer Vrije Universiteit Brussel

Bharat Jasani, Gudrun Bänfer, Rebecca Fish, Wim Waelput, Yves Sucaet, Craig Barker, Jessica L. Whiteley, Jill Walker, Rudy Hovelinck, Rolf Diezko, et al.

BACKGROUND: Numerous studies indicate that higher tumour programmed cell death ligand-1 (PD-L1) expression is associated with greater response to anti-programmed cell death-1 (PD-1)/PD-L1 immunotherapy in non-small cell lung cancer (NSCLC). In the era of precision medicine, there is a need to provide reliable, standardised training for pathologists to improve their accuracy of interpretation and scoring, as the results are used directly to ...

Evaluation of Distance Metrics and Spatial Autocorrelation in Uniform Manifold Approximation and Projection Applied to Mass Spectrometry Imaging Data Vrije Universiteit Brussel KU Leuven

Tina Smets, Marc Claesen, Thomas Tousseyn, Wim Waelput
In this work, uniform manifold approximation and projection (UMAP) is applied for nonlinear dimensionality reduction and visualization of mass spectrometry imaging (MSI) data. We evaluate the performance of the UMAP algorithm on MSI data sets acquired in mouse pancreas and human lymphoma samples and compare it to those of principal component analysis (PCA), t-distributed stochastic neighbor embedding (t-SNE), and the Barnes-Hut (BH) ...

Early-Onset Creutzfeldt-Jakob Disease Mimicking Immune-Mediated Encephalitis Vrije Universiteit Brussel

Wietse Wiels, Stephanie Du Four, Laura Seynaeve, Anja Flamez, Thomas Tousseyn, Dietmar Thal, Miguel D'Haeseleer

Objectives: The objective of this study is to explore the clinical, radiological, and pathological manifestations of a rare subtype of prion disease and their implication for differential diagnosis in case of an early onset neuropsychiatric deterioration.

Methods: We discuss a patients' clinical history, as well as the string of investigations and symptomatological evolution that finally led to a pathological diagnosis.

Results: ...