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Site-selective functionalization of nanobodies using intein-mediated protein ligation for innovative bioconjugation

Boekbijdrage - Hoofdstuk

An expression strategy is presented in order to produce nanobodies modified with a clickable alkyne function at their C-terminus via the Intein-mediated Protein Ligation (IPL) technique. The protocol focuses on the cytoplasmic expression and extraction of a nanobody-intein-chitin binding domain (CBD) fusion protein in E. coli SHuffle® T7 cells, in the commonly used Luria-Bertani (LB) medium. The combination of these factors results in a high yield and nearly complete alkynation of the nanobody at its C-terminus via IPL. The resulting alkynated nanobodies retain excellent binding capacity towards the nanobody targeted antigen. The presented protocol benefits from time- and cost-effectiveness and allows a feasible up-scaling of functionalized (here alkynated) nanobodies. The production of high quantities of site-specifically modified nanobodies paves the way to i) novel biosurface applications that demand for homogeneously oriented nanobodies having their active site fully accessible for target (e.g. biomarker) binding, and ii) innovative applications such as localized drug delivery and image guided surgery by covalent ‘click’ chemistry coupling of these alkynated nanobodies to a multitude of azide-containing counterparts as there are drug containing polymers and contrast labeling agents.
Boek: Bioconjugation: Methods and Protocols
Series: Methods in Molecular Biology
Pagina's: 117 - 130
ISBN:9781493996537
Jaar van publicatie:2019
Trefwoorden:Protein functionalization, intein, nanobody, click chemistry