Production of antibody derivatives in the methylotrophic yeast Pichia pastoris

New antibody derivatives are continuously being generated to interact with a range of therapeutic targets. The cost-effective and efficient production of these and other antibody derivatives is crucial for their further success. Here, we describe the construction of the expression vectors needed for heterologous expression of a Fab fragment in the yeast Pichia pastoris. The experimental conditions for lab-scale expressions are discussed, and an overview of an efficient purification strategy is presented.

ISBN:978-1-61779-973-0

Jaar van publicatie:2012

Trefwoorden:Antibody production, Antibody derivatives, Yeast expression, Pichia pastoris