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Optimization of a Nile Red method for rapid lipid determination in autotrophic, marine microalgae is species dependent

Tijdschriftbijdrage - Tijdschriftartikel

Several studies have been conducted to develop rapid methods for quantification of lipid content in microalgae,as an alternative for time consuming gravimetric methods. Different studies showed that lipid stainingwith NileRed in whole cell suspensions and subsequently quantification by the use of a spectrofluorometric device is apromising method, but a profound optimization and validation is rare. It has already been proven that the correlationcurve for quantification is species dependent, but it has not yet been investigated whether this is also thecase for the optimization of the Nile Red assay protocol. Therefore, two autotrophic, marine microalgae,Nannochloropsis oculata and T-Isochrysis lutea, strongly differing in e.g. cell wall structure, were selected in thisstudy to investigatewhether optimization of the Nile Red assay is species dependent. Besides this, it was checkedfor one of these species, Nannochloropsis, whether the lipid content, determined by the Nile Red assay, could indeedbe correlated with the neutral and/or total lipid content determined by gravimetric methods.Itwas found that optimization of the Nile Red assay was strongly species dependent. Consequently, optimizationhas to be done for each species before using the assay. For Nannochloropsis, a good correlation was found betweentotal and neutral lipid content obtained by the Nile Red assay and by gravimetric methods.
Tijdschrift: Journal of Microbiological Methods
ISSN: 0167-7012
Volume: 118
Pagina's: 152 - 158
Jaar van publicatie:2015