Achieving a Peak Capacity of 1800 Using an 8 m Long Pillar Array Column

In the present study, the peak capacity potential of ultralong porous cylindrical pillar array columns is investigated. Coupling 4 columns of 2 m long allows for working near the minimal separation impedance of small molecules under retained conditions at a maximal pressure load of 250 bar. Minimal plate heights of H = 5.0 mu m, H = 6.3 mu m, and H = 7.7 mu m were obtained for uracil (unretained), butyrophenone (k = 0.85), and valerophenone (k = 1.94), respectively, corresponding to a number of theoretical plates of N = 1.6 X 10(6), N = 1.2 x 10(6), and N = 1.0 x 10(6). The optimal linear velocities were 0.60 mm/s for a retained compound and 0.74 mm/s for an unretained compound. Based on a mixture of 9 compounds, the peak capacity tic was determined as a function of gradient time (t(G)). Peak capacities (t(G)-based) of 1103 and 1815 were obtained when applying 650 min and 2050 min gradients (t(G)/t(0) = 4.5 and 14, respectively, with t(G) as the gradient time and t(0) as the void time). These values are much higher than earlier reported peak capacity values for small molecules.

Jaar van publicatie:2019

Trefwoorden:OPTIMAL MIXING RATE; LIQUID-CHROMATOGRAPHY; MONOLITHIC COLUMNS; CAPILLARY; PHASE; OPTIMIZATION; PERFORMANCE; RESOLUTION; SEPARATIONS; EFFICIENCY

CSS-citation score:2

Toegankelijkheid:Open