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Measurement of albumin secretion as functionality test in primary hepatocyte cultures
Boekbijdrage - Hoofdstuk
One of the most important functions of hepatocytes is the synthesis of
serum proteins, more specifically of serum albumin. Albumin secretion
in serum is essential, since it maintains the oncotic pressure in the
body. Measurement of albumin secretion is used as a liver function test
to indicate potential liver injury and liver pathology. In this chapter, a
protocol for the measurement of albumin secretion in the supernatant of
cultured rat hepatocytes is described. The procedure relies on an enzymelinked
immunosorbent assay allowing rat albumin to be quantitatively
measured.
serum proteins, more specifically of serum albumin. Albumin secretion
in serum is essential, since it maintains the oncotic pressure in the
body. Measurement of albumin secretion is used as a liver function test
to indicate potential liver injury and liver pathology. In this chapter, a
protocol for the measurement of albumin secretion in the supernatant of
cultured rat hepatocytes is described. The procedure relies on an enzymelinked
immunosorbent assay allowing rat albumin to be quantitatively
measured.
Boek: Protocols in In Vitro Hepatocyte Research
Series: Methods in Molecular Biology
Pagina's: 303-308
Aantal pagina's: 6
ISBN:978-1-4939-2073-0
Jaar van publicatie:2015
Trefwoorden:albumin, hepatocyte, liver, ELISA