Developing a versatile genome editing tool for C. glutamicum to enhance chito-oligosaccharides production

Chito-oligosaccharides are a class of molecules, composed of N-acetyl glucosamine and glucosamine sub-units, that possess various interesting properties. Currently, progress towards microbial in vivo production of these molecules was conducted in Escherichia coli. A disadvantage using E. coli may be its sub-optimal background metabolism. However, other production hosts that might harbor a more suitable metabolic basis tend to have a limited genetic toolbox, e.g. Corynebacterium glutamicum. Here we propose a versatile and efficient genome editing tool for C. glutamicum in order to substantially increase its in vivo production of specific chito-oligosaccharides.