Time-resolved cryo-EM using a combination of droplet microfluidics with on-demand jetting Vrije Universiteit Brussel Flanders Institute for Biotechnology
Single-particle cryogenic electron microscopy (cryo-EM) allows reconstruction of high-resolution structures of proteins in different conformations. Protein function often involves transient functional conformations, which can be resolved using time-resolved cryo-EM (trEM). In trEM, reactions are arrested after a defined delay time by rapid vitrification of protein solution on the EM grid. Despite the increasing interest in trEM among the ...