In vivo Directed Evolution of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in Saccharomyces cerevisiae Using an Orthogonal DNA Replication System KU Leuven
To create an efficient and effective solar fuels process that combines solar electricity with biological organisms, any participatory enzymes in the cells need to have sufficiently high turnover rates to match the input of solar energy. A substantial breakthrough in this regard would be to develop an improved RuBisCO enzyme, which fixes CO2 from the atmosphere, with enhanced turnover rate and selectivity. In nature, RuBisCO has slow turnover ...