Target identification for orphan peptides through furan-oxidation based cross-linking for application in cancer research Ghent University
Furan-modified peptides will be synthesized for cross-linking to known and unknown target receptors.
Furan-modified peptides will be synthesized for cross-linking to known and unknown target receptors.
In the current project we will use UGent technology (UGent IP) to provide an industrial proof-of-concept for its use in the development of an ELISA assay with substantially increased sensitivity. During the project there will be close collaboration with a Flemish Biotech company that is active in the IVD market. Company X will be granted a license option in their domain, while through this interdisciplinary approach Ugent will be given the ...
Using a variety established DNA crosslinking nucleobase-analogues a method will be developed for the stapling of the 5’ and 3’ ends of G-quadruplex forming aptamer sequences. This staple will in theory convey several benefits such as conformational locking and improved formation kinetics. Crosslinked aptamers will then be assessed for improvements in these properties compared to wild type oligonucleotides.
This project aims at synthesizing furan-modified aptamers able to selectively crosslink to their cell surface targets. The furan group allows spatiotemporal control of the crosslinking reaction, as only activated furan can form covalent bonds with the target. Selective activation is achieved by the production of singlet oxygen or reactive oxygen species. Thus, we envisage the production of high affinity covalent ligands for cell-surface ...
Furan-modified oligonucleotides and oligopeptides are used in DNA-DNA and DNA-peptide covalent crosslinking. Formation of a site specific, permanent crosslink is of biological importance in the study of DNA repair and cancer treatment. Molecular dynamics and ab-inito quantum chemical (QMMM) calculations, together with NMR prediction will be used to explain and support the experimental work and guide the design of new modifications.
Trough synthesis of major groove binding peptides incorporating an unnatural furan-amino acid building block, we will try to achieve DNA-peptide cross-linking. More specificallay, we have chosen to work on a modelsystem that was developed at Kyoto University and that can ben synthetically modified with the desired crosslinking functionality. After complexation of peptide with the DNA-duplex, the furan moeity will be oxidized to a reactive ...
Through synthesis of major groove binding peptides incorporating an unnatural furan-amino acid building block, we will try to achieve DNA-peptide cross-linking. After complexation of peptide with DNA-duplex, the furan moeity will be oxidized to a reactive enal functionality. Subsequent reaction with the nucleophilic exocyclic amines of DNA bases in major groove will lead to the desired cross-link reaction.
Exploiting the use of TAD-based reversible (cross)linking in polymer based systems
For reasons of confidentiality the project summary cannot be disclosed.
For more information please contact the (co)promoter.