Publications
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Using BRET-based G protein biosensors to unravel the G protein mediated pathways in insects KU Leuven
G protein-coupled receptors (GPCRs) are membrane-bound receptors which are candidate targets for novel insect pest management strategies. The superfamily of GPCRs is extensively studied in vertebrates since they are pharmacological targets for many important therapeutic compounds. However, in insects, the molecular signaling properties of GPCRs remain poorly understood and studies are usually limited to the detection of fluctuations in the ...
Design of novel refractive-index biosensors based on dielectric one-dimensional photonic crystals combined with graphene Vrije Universiteit Brussel
Biosensors are monitoring devices that are currently used in a wide range of applications, such as in the measurement of blood glucose levels or the concentration of water pollutants, in DNA sequencing processes, and many more. While many classes of biosensors exist, one type of sensors, the label-free refractive-index optical biosensors, received a great deal of attention in recent years due to their high sensitivity, compactness, robustness, ...
Nanometer-thick Mn:NiO and Co:NiO films for high performance nonenzymatic biosensors University of Antwerp
People with diabetes require regular blood sugar level monitoring, using commercial enzyme-based biosensors. There is a considerable need to develop biosensors with nonenzymatic electrodes to eliminate the drawbacks of enzymes. Nanostructured nickel oxide (NiO) thin films are highly promising materials for the development of nonenzymatic glucose and hydrogen peroxide (H2O2) biosensors. Although the biosensor performance can be easily attained ...
2D nanomaterials as the basis for DNA biosensors with SNP detection capabilities Hasselt University
Developments in biosensing techniques have provided a wide range of diagnostic solutions in recent years for a variety of sectors such as medicine, agriculture and biotechnology. The subject is so broad in fact, that only a small fragment of one of the main categories, namely label-free electrochemical sensors, will be discussed in the present thesis. The development of a highly selective and sensitive biosensor platform for SNPs would be a ...
Recent advances in electrochemical biosensors based on fullerene-$C_{60}$ nano-structured platforms University of Antwerp
Nanotechnology is becoming increasingly important in the field of (bio)sensors. The performance and sensitivity of biosensors is greatly improved with the integration of nanomaterials into their construction. Since its first discovery, fullerene-C60 has been the object of extensive research. Its unique and favorable characteristics of easy chemical modification, conductivity, and electrochemical properties has led to its tremendous use in ...
REGA-SIGN: Development of a Novel Set of NanoBRET-Based G Protein Biosensors KU Leuven
Despite G protein-coupled receptors (GPCRs) being important theapeutic targets, the signaling properties of many GPCRs remain poorly characterized. GPCR activation primarily initiates heterotrimeric G protein signaling. To detect ligand-induced G protein activation, Bioluminescence Resonance Energy Transfer (BRET)-based biosensors were previously developed. Here, we designed a novel set of Nanoluciferase (NLuc) BRET-based biosensors (REGA-SIGN) ...
Smart genetically-encoded biosensors for the chemical monitoring of living systems KU Leuven
Genetically-encoded biosensors provide the all-optical and non-invasive visualization of dynamic biochemical events within living systems, which has allowed the discovery of profound new insights. Twenty-five years of biosensor development has steadily improved their performance and has provided us with an ever increasing biosensor repertoire. In this feature article, we present recent advances made in biosensor development and provide a ...
Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification KU Leuven
Genetically-encoded biosensors based on a single fluorescent protein are widely used to visualize analyte levels or enzymatic activities in cells, though usually to monitor relative changes rather than absolute values. We report photochromism-enabled absolute quantification (PEAQ) biosensing, a method that leverages the photochromic properties of biosensors to provide an absolute measure of the analyte concentration or activity. We develop ...
Enhancing the Performance of DNA Surface-Hybridization Biosensors through Target Depletion Hasselt University KU Leuven
DNA surface-hybridization biosensors utilize the selective hybridization of target sequences in solution to surface-immobilized probes. In this process, the target is usually assumed to be in excess, so that its concentration does not significantly vary while hybridizing to the surface-bound probes. If the target is initially at low concentrations and/or if the number of probes is very large, and they have high affinity for the target, the DNA ...