Publications
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The expression of hepatic transport proteins in progenitor cells and liver tumours : diagnostic and pathogenetic implications KU Leuven
Levertransporteiwitten zijn betrokken in belangrijke fysiologische funct ies van de lever door het transport van een brede waaier van endogene en exogene substraten over de celmembraan. De aanwezigheid van leden van d e ATP-binding cassette (ABC) transportfamilie in tumoren, zorgen ervoor dat een brede waaier van structurele en functioneel ongerelateerde chemo therapeutica uit de kankercel worden getransporteerd zodat de cellen res istent worden ...
Biological and physicochemical characterization of a serum- and xeno-free chemically defined cryopreservation procedure for adult human progenitor cells University of Antwerp
While therapeutic cell transplantations using progenitor cells are increasingly evolving towards phase I and II clinical trials and chemically defined cell-culture is established, standardization in biobanking is still in the stage of infancy. In this study, the EU FP6-funded CRYSTAL (CRYo-banking of Stem cells for human Therapeutic AppLications) consortium aimed to validate novel Standard Operating Procedures (SOPs) to perform and validate ...
The Beta-Adrenoceptor Agonist Isoproterenol Rescues Acetaminophen-Injured Livers Through Increasing Progenitor Numbers by Wnt in Mice KU Leuven
UNLABELLED: Acetaminophen (APAP)-induced acute liver injury (AILI) is a major health problem. Accumulating evidence suggests that the sympathetic nervous system (SNS) regulates neuronal and hematopoietic progenitors. SNS signaling affects hepatic progenitor/oval cells (HPCs) and β-adrenoceptor agonism will expand HPCs to reduce AILI. Dopamine β-hydroxylase-deficient mice (Dbh-/-), lacking catecholamine SNS neurotransmitters, isolated HPCs, and ...
Osteopontin neutralisation abrogates the liver progenitor cell response and fibrogenesis in mice Vrije Universiteit Brussel
Background Chronic liver injury triggers a progenitor cell repair response, and liver fibrosis occurs when repair becomes deregulated. Previously, we reported that reactivation of the hedgehog pathway promotes fibrogenic liver repair. Osteopontin (OPN) is a hedgehog-target, and a cytokine that is highly upregulated in fibrotic tissues, and regulates stem-cell fate. Thus, we hypothesised that OPN may modulate liver progenitor cell response, and ...
Role of BMP Signaling in Pancreatic Progenitor Differentiation from Human Embryonic Stem Cells Vrije Universiteit Brussel
Transplantation of pancreatic progenitors derived from human embryonic stem cells (hESCs) is a promising way to treat diabetes. Strategies to obtain the required cell mass would rely on the up scaling of current differentiation protocols, or the proliferation of committed progenitors. We aimed at finding conditions that maintain a proliferating pancreatic progenitor pool and we assessed the role of BMP4 signaling in this process. hESCs were ...
Diversity in striatal synaptic circuits arises from distinct embryonic progenitor pools in the ventral telencephalon University of Antwerp
Synaptic circuits in the brain are precisely organized, but the processes that govern this precision are poorly understood. Here, we explore how distinct embryonic neural progenitor pools in the lateral ganglionic eminence contribute to neuronal diversity and synaptic circuit connectivity in the mouse striatum. In utero labeling of T alpha 1-expressing apical intermediate progenitors (aIP), as well as other progenitors (OP), reveals that both ...
Pituitary Progenitor Cells Tracked Down by Side Population Dissection KU Leuven University of Antwerp
Sca1(high) subset within the SP, stem cell growth factors that induce SP expansion, affect transcription of embryonic factors, suggesting impact on a developmental program that unfolds within this SP compartment. Non-Sca1(high) SP cells, revealed by Sox2 expression, are observed in the postulated periluminal stem/progenitor cell niche, but also in small groups scattered over the gland, thereby advocating the existence of multiple niches. In ...
Microcarrier Screening and Evaluation for Dynamic Expansion of Human Periosteum-Derived Progenitor Cells in a Xenogeneic Free Medium KU Leuven
An increasing need toward a more efficient expansion of adherent progenitor cell types arises with the advancements of cell therapy. The use of a dynamic expansion instead of a static planar expansion could be one way to tackle the challenges of expanding adherent cells at a large scale. Microcarriers are often reported as a biomaterial for culturing cells in suspension. However, the type of microcarrier has an effect on the cell expansion. In ...
Urine of Preterm Neonates as a Novel Source of Kidney Progenitor Cells KU Leuven
In humans, nephrogenesis is completed prenatally, with nephrons formed until 34 weeks of gestational age. We hypothesized that urine of preterm neonates born before the completion of nephrogenesis is a noninvasive source of highly potent stem/progenitor cells. To test this hypothesis, we collected freshly voided urine at day 1 after birth from neonates born at 31-36 weeks of gestational age and characterized isolated cells using a single-cell ...