Projects
Recombinant expression of RNAi by means of selected candidate vaccine antigens of Ostertagia ostertagi in Caenorhabditis elegans Ghent University
Study of neuroblastoma oncogenesis through mRNA and miRNA expression profiling of normal neuroblasts and neuroblastoma stem cells Ghent University
Neuroblastoma is a tumor of embryonal origin with a clinical variable outcome. Given the low survival rates of a subset of patients with neuroblastoma, there is an urgent need for new therapies. In this project, we aim to identify new therapeutic targets of neuroblastoma through mRNA and miRNA expression profiling of human and murine progenitor cells. Through integrated molecular profiling we aim to identify new neuroblastoma candidate genes ...
Design and use of a novel HEK293S-based expression system for eukaryotic (membrane) proteins Ghent University
HEK293 cells are genetically modified in such a way that they become better adapted to express and accomodate membrane proteins.
Gene expression Ghent University
Understanding gene expression at a systems level is crucial for gaining correct insights in development and disease of complex organisms. Transcription regulatory networks describe gene expression as a function of inputs specified by physical and funcitonal interactions between transcription factors, miRNAs, cofactors and DNA. We investigate reverse-engineering algorithms to more correctly infer transcription regulatory networks from ...
Differential gene expression of intestinal dendritic cells after (in vivo) interaction with soluble immunogenic or tolerogeneic antigens Ghent University
Dendritic cells will be isolated from the small intestine of weaned piglets after stimulation with soluble immunogenic and tolerogeneic antigens. The influence of these antigens on the maturation of intestinal dendritic cells will be analysed by flowcytometric analysis of co-stimulatory molecules and analysis of cytokine/chemokine gene expression.
Influence of gastric banding and exercise training on the expression of "Sterol regulatory element-binding proteins" (SREBPs) in the skeletal muscle and his association to intramyocitic triglycerides in morbid obese patients Ghent University
The mechanisms of the increased intramyocitic triglycerides (IMTG) in insulin resistance and exercise training are not known. A possible pathway could be found in the expression of SREBPs. The purpose of this study is to investigate the effect of gastric banding and exercise training on the expression of SREBPs and its relation to IMTG content in morbidly obese patients.