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Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production. KU Leuven
The choice of an expression system for the metagenomic DNA of interest is of vital importance for the detection of any particular gene or gene cluster. Most of the screens to date have used the Gram-negative bacterium Escherichia coli as a host for metagenomic gene libraries. However, the use of E. coli introduces a potential host bias since only 40% of the enzymatic activities may be readily recovered by random cloning in E. coli. To recover ...
Cloning and expression of metagenomic DNA in Streptomyces lividans and subsequent fermentation for optimized production KU Leuven
The choice of an expression system for the metagenomic DNA of interest is of vital importance for the detection of any particular gene or gene cluster. Most of the screens to date have used the gram-negative bacterium Escherichia coli as a host for metagenomic gene libraries. However, the use of E. coli introduces a potential host bias since only 40 % of the enzymatic activities may be readily recovered by random cloning in E. coli. To recover ...
Cloning and expression of metagenomic DNA in Streptomyces lividans and subsequent fermentation for optimized production KU Leuven
The choice of an expression system for the metagenomic DNA of interest is of vital importance for the detection of any particular gene or gene cluster. Most of the screens to date have used the gram-negative bacterium Escherichia coli as a host for metagenomic gene libraries. However, the use of E. coli introduces a potential host bias since only 40 % of the enzymatic activities may be readily recovered by random cloning in E. coli. To recover ...
Computational and Omics Analyses providing new Leads for improving Streptomyces lividans for Heterologous Protein Production KU Leuven
As the industrial and medical use of enzymes intensifies, so does the search for appropriate host organisms to produce these enzymes heterologously. In this respect, the bacterium Streptomyces lividans is increasingly attracting attention, mainly because of its favorable traits concerning protein secretion. This dissertation encompasses a contribution to the scientific community's efforts to understand and modify S. lividans in order to prepare ...
Cloning and expression vectors for a Gram-positive host, Streptomyces lividans KU Leuven
The choice of an expression system for the meta-genomic DNA of interest is of vital importance for the detection of any particular gene or gene cluster. Most of the screens to date have used the Gram-negative bacterium Escherichia coli as a host for the meta-genomic gene libraries. However, the use of E. coli introduces a potential host bias since only 40% of the enzymatic activities may be readily recovered by random cloning in E. coli (Gabor ...
Production and Assessment of Antioxidant Activity of Exopolysaccharide from Marine Streptomyces globisporus BU2018 KU Leuven
MICROBIAL polysaccharides are involved in a wide range of biotechnological applications. Three streptomycetes isolates isolated from marine sediment from Sharm El-Sheikh were screened for their ability to produce exopolysaccharides (EPSs) as well as the antioxidant activity. Streptomyces globisporus BU2018 producing EPS which had the highest antioxidant activity was identified based on morphological, biochemical characteristics as well as ...
Immune response to Streptomyces lividans in mice: a potential vaccine vehicle against TB KU Leuven
The potentialities of Streptomyces lividans 1326 as new live vaccine vehicle strategy have been evaluated. Immunization of mice with the Streptomyces mycelium induced high levels of specific antibodies against proteins released in the culture supernatant of the analyzed strain. Splenocytes from the Streptomyces-immunized animals were able to secrete high levels of IFN-γ (2103.9 pg/mL) and to proliferate in vitro on stimulation with proteins ...
Use of Strep-tagII for rapid detection and purification of Mycobacterium tuberculosis recombinant antigens RV2626c and RV3804 in Streptomyces lividans KU Leuven
Recent results with respect to the secretory production of bio-active Mycobacterium tuberculosis proteins in Streptomyces have stimulated the further exploitation of this host as a bacterial cell factory. However, the rapid isolation of a recombinant protein by conventional procedures can be a restrictive step. A previous attempt to isolate recombinant antigens fused to the widely used 6His-tag was found to be relatively incompatible with ...