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Gene expression profiling of early hepatic stellate cell activation reveals a role for Igfbp3 in cell migration Vrije Universiteit Brussel KU Leuven
BACKGROUND: Scarring of the liver is the result of prolonged exposure to exogenous or endogenous stimuli. At the onset of fibrosis, quiescent hepatic stellate cells (HSCs) activate and transdifferentiate into matrix producing, myofibroblast-like cells.
AIM AND METHODS: To identify key players during early HSC activation, gene expression profiling was performed on primary mouse HSCs cultured for 4, 16 and 64 hours. Since valproic acid ...
A 3′‐end capture sequencing method for high‐throughput targeted gene expression profiling Ghent University
Molecular phenotyping through shallow 3 '-end RNA-sequencing workflows is increasingly applied in the context of large-scale chemical or genetic perturbation screens to study disease biology or support drug discovery. While these workflows enable accurate quantification of the most abundant genes, they are less effective for applications that require expression profiling of low abundant transcripts, like long noncoding RNAs (lncRNAs), or ...
Gene expression profiling as a tool to evaluate immune responses University of Antwerp
The human immune system is a very complex defense mechanism, consisting of different levels of interacting cells and molecules, to keep out and eliminate pathogens and foreign particles. The immune system can be divided into three main categories: 1) the physical barriers, 2) the innate immune system that creates immediately non-pathogen-specific immune responses, and 3) the adaptive immune system that can activate pathogen-specific immune ...
Transcriptome profiling in blood before and after hepatitis B vaccination shows significant differences in gene expression between responders and non-responders Hasselt University Vrije Universiteit Brussel University of Antwerp
Introduction: As the hepatitis B virus is widely spread and responsible for considerable morbidity and mortality, WHO recommends vaccination from infancy to reduce acute infection and chronic carriers. However, current subunit vaccines are not 100% efficacious and leave 5-10% of recipients unprotected. Methods: To evaluate immune responses after Engerix-B vaccination, we determined, using mRNA-sequencing, whole blood early gene expression ...
Gene expression profiling and molecular signaling of various cells in response to tricalcium silicate cements : a systematic review Ghent University
Comparison of a gene expression profiling strategy to standard clinical work-up for determination of tumour origin in cancer of unknown primary (CUP) Vrije Universiteit Brussel
CupPrint® is a genomic signature able to identify 47 different cancer types. The aim of our study was to compare the accuracy of this genomic signature to that of a full clinical work-up in diagnosing the primary tumour site. Patients with newly diagnosed, untreated metastatic tumours were eligible for this trial. The clinical work-up and gene expression profiling on a biopsy from a metastatic site were started at the same time. The study was ...
Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel KU Leuven University of Antwerp
Background: Genomic and transcriptomic approaches have the potential for unveiling the genome-wide response to environmental perturbations. The abundance of the catadromous European eel (Anguilla anguilla) stock has been declining since the 1980s probably due to a combination of anthropogenic and climatic factors. In this paper, we explore the transcriptomic dynamics between individuals from high (river Tiber, Italy) and low pollution (lake ...