Phosphorylases as cost-efficient biocatalysts for carbohydrate synthesis: an exploration of natural and mutant diversity

Glycoside phosphorylases are able to synthesize specialty carbohydrates starting from cheap glycosyl phosphates as donor substrates. Unfortunately, the number of available specificities currently is very limited and their products are rarely relevant for the industry. This portfolio will, therefore, be significantly expanded through a combination of state-of-the-art techniques in database mining and enzyme engineering.