Pharmacokinetics of fluoroquinolones in critical care patients: A bio-analytical HPLC method for simultaneous quantification of ofloxacin, ciprofloxacin and moxifloxacin in human plasma Ghent University
A high-performance liquid chromatographic (HPLC) method with fluorescence detection was developed and validated for the simultaneous quantification of ofloxacin, ciprofloxacin and moxifloxacin in human plasma. Sarafloxacin was used as internal standard. Chromatography was carried out using a Waters XBridge (TM) C-18 HPLC column and a gradient mobile phase consisting of CH3CN/MeOH/0.025 M TBA center dot Cl/TFA (eluent A at 75/25/899/1 (v/v); ...