Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR

Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is a sensitive technique for quantifying gene expression levels. One or more appropriate reference genes must be selected to accurately compare mRNA transcripts across different samples and tissues. Thus far, only actin-2 has been used as a reference gene for qRT-PCR in chicory, and a full comparison of several candidate reference genes in chicory has not yet been reported.

Publication year:2010

Keywords:Actins, Chicory, Gene Expression Profiling, Genes, Plant, Peptide Elongation Factor 1, Plant Leaves, Plant Roots, Polymerase Chain Reaction, RNA, Ribosomal, 18S, Reference Standards