Development of a liquid chromatographic method for the determination of related substances and assay of D-cycloserine

D-cycloserine or D-4-amino-3-isoxazolidinone is an antibiotic produced by Streptomyces garyphalus and Streptomyces orchidaceus. D-cycloserine is used in the second line treatment of tuberculosis and is often used in developing countries. Therefore, expensive high-tech techniques are not recommended for analysis. Here, a liquid chromatography method with ultraviolet detection (LC-UV) is described using a base deactivated column (Hypersil BDS column; 25 cm x 4.6 mm I.D.) kept at 45 degrees C. The gradient method uses mobile phases containing acetonitrile (ACN), 20 mM sodium octane sulphonate (SOS), 0.2 M potassium dihydrogen phosphate buffer pH 2.8, water: A: (4:70:10:16 v/v/v/v) and 13: (17:70:10:3 v/v/v/v). The method proved to be robust, linear, repeatable, sensitive, selective and easy to perform. For the related substances test 50 mu l of a 0.5 mg/ml D-cycloserine solution is injected. For assay, a concentration of 0.1 mg/ml is proposed to avoid overloading of the detector. (C) 2008 Elsevier B.V. All rights reserved.

Publication year:2008

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Authors from:Higher Education