High-throughput multiplex quantitative PCR

Quantitative PCR (qPCR) is a mainstay of genomic research: it enables a range of analyses that complement or support genome-, transcriptome- and epigenome-wide analyses. qPCR is required to accurately calibrate mixtures of sequencing library, to assess expression of selected genes in a multitude of biological samples, to quantify locus-specific DNA methylation in a bisulfite-independent manner, to measure the level of histone modifications at prespecified regions, to confirm DNA copy number changes in DNA samples with putative aneuploidies and for a range of other applications. While originally developed to measure a single target at a time, advances in the past decades have enabled the analysis of up to 5 targets in a single reaction. Given the pivotal nature of these analyses and the growing need for accurate qPCR measurements in the expanding genomic research, we here request a state-of-the-art qPCR instrument with the capability to analyze multiplexed reactions.

Keywords:(epi)genomics, multiplex quantitative PCR, transcriptomics, biomarker research, genotyping

Disciplines:Epigenetics, Genetics, DNA analysis technology, Medical biotechnology diagnostics