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Publication
An RNA in situ hybridization protocol optimized for monocot tissue
Journal Contribution - Journal Article
RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense probes for each gene of interest and hybridize them to tissue sections.
Journal: STAR PROTOCOLS
ISSN: 2666-1667
Issue: 2
Volume: 2
Publication year:2021
Accessibility:Open