Pathogenetic role of endothelial nitric oxide synthase uncoupling in ischemia- and reperfusion injury of the lung.

The objective of our research is to determine whether the process of eNOS uncoupling can also be demonstrated in lung tissue after ischemia and reperfusion injury (IRI). In the first part of the project, experimental techniques were developed in order to detect free radical production during IRI using electron paramagnetic resonance. A murine model of pulmonary IRI was also developed. We are currently working on the effect of eNOS itself, and eNOS uncoupling, on free radical generation during pulkmonary IRI. Our final aim is to develop therapeutic strategies to tackle pulmonary IRI in patients undergoing complex surgery such as cardiopulmonary bypass, lung transplantation and isolated lung perfusion.

Keywords:ENDOTHELIAL CELLS, ISCHEMIA/REPERFUSION

Disciplines:Microbiology, Systems biology, Laboratory medicine

Project type:Collaboration project