Biomimetic remineralization of caries affected dentin

Biomimetic remineralization is a technique to grow dental minerals, namely hydroxyapatite, in form shape and mechanical properties similar to the host material dentin. Primarily this can be achieved by phosphorylation of demineralized type I collagen, followed by exposure to Ca2+. Clinically this may be very useful in the prevention and treatment of dentin caries and to create a biocompatible barrier to protect the iatrogenically exposed pulp. Recent studies have shown that it is possible to remineralize demineralized dentin surfaces. Even some clinical evidence is available in the treatment of dentin hypersensitivity. However, no data are available regarding protocols that could be applicable in clinic. The purpose is to develop/select materials that in a clinical-like cavity model can repair defects by biomimetic remineralization of demineralized dentin itself and in a later stage of artificial scaffolds. In a pilot study the feasibility was assessed: a micro-cavity design was used to assess the effect of Portland cement (Biodentine, Septodont) on normal dentin. Using correlative analysis at high spatial resolution we could track all transfers and minute changes on an elemental (EPMA), chemical (micro-Raman) and ultra-morphological (TEM) level. The proposed research project is a logical continuation of the pilot study that will expand the KU Leuven BIOMAT expertise to a new field of resin-free dental restorative materials.