Influence of melphalan isolated lung perfusion on the (phospho)-proteome.

This project studies the regulation, phosphorylation and adduct formation of proteins in tumors upon melphalan treatment. LC-MS methods will be developed using a representative cell culture. An animal model (induced lung tumors) will be treated by melphalan isolated lung perfusion prior to analysis of human samples. Final goal is the selection of candidate biomarkers ((phospho-)protein, melphalan-proteinadduct, ...) with prognostic value for the treatment of lung metastases by melphalan-ILuP.

Keywords:PROTEOME ANALYSIS, MASS SPECTROMETRY, LUNG METASTASEN, ISOLATED PULMONARY PERFUSION

Disciplines:Analytical chemistry, Morphological sciences, Oncology, Pharmaceutical analysis and quality assurance