Cells producing glycoproteins having altered N- and O-glycosylation patterns and methods and use

The present application relates to the field of glyco-engineering, more specifically to eukaryotic cells wherein both an endoglucosaminidase is present, and made deficient in UDP-galactose 4-epimerase (GalE). Typically, a glycoprotein is also present in the cells. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular without the need for adding an extra enzyme. Methods are also provided for the application of these cells in protein production.

Patent Publication Number: EP3320105

Year filing: 2021

Year approval: 2021

Year publication: 2021

Status: Assigned

Technology domains: Biotechnology

Validated for IOF-key: Yes

Attributed to: Associatie Universiteit Gent