Extracellular vesicles from follicular and ampullary fluid isolated by density gradient ultracentrifugation improve bovine embryo development and quality Universiteit Antwerpen Universiteit Gent
Extracellular vesicles (EVs) have been isolated from follicular (FF) and ampullary oviduct fluid (AOF), using different isolation methods. However, it is not clear whether different purification methods can affect the functionality of resulting EVs. Here, we compared two methods (OptiPrep (TM) density gradient ultracentrifugation (ODG UC) and single-step size exclusion chromatography (SEC) (qEV IZON (TM) single column)) for the isolation of EVs ...