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Govania unica gen. nov., sp. nov., a rare biosphere bacterium that represents a novel family in the class Alphaproteobacteria Universiteit Gent
Strain LMG 31809 T was isolated from a top soil sample of a temperate, mixed deciduous forest in Belgium. Comparison of its 16S rRNA gene sequence with that of type strains of bacteria with validly published names positioned it in the class Alphaproteobacteria and highlighted a major evolutionary divergence from its near neighbor species which represented species of the orders Emcibacterales and Sphingomonadales. 16S rRNA amplicon sequencing of ...
Taming CRISPRi : dynamic range tuning through guide RNA diversion Universiteit Gent
CRISPRi is a powerful technique to repress gene expression in a targeted and highly efficient manner. However, this potency is a double-edged sword in inducible systems, as even leaky expression of guide RNA results in a repression phenotype, complicating applications such as dynamic metabolic engineering. We evaluated three methods to enhance the controllability of CRISPRi by modulating the level of free and DNA-bound guide RNA complexes. ...
Detectie van sporen van de productie van synthetisch drugs in oppervlaktewater Universiteit Gent
Wereldwijd behoren amfetamine, methamfetamine en ecstasy tot de meest gebruikte illegale drugs na cannabis en cocaïne. Hun gebruik is drastisch gestegen in de jaren 90 maar afgelopen jaren werd een lagere groei vastgesteld. Naast de productie en consumptie van deze synthetische drugs, krijgt de politie ook te maken met het illegaal lozen van afval afkomstig uit het productieproces van deze drugs. Het doel van voorliggend onderzoek was om aan de ...
MoBioS : modular platform technology for high-throughput construction and characterization of tunable transcriptional biological sensors Universiteit Gent
All living organisms have evolved and fine-tuned specialized mechanisms to precisely monitor a vast array of different types of molecules. These natural mechanisms can be sourced by researchers to build Biological Sensors (BioS) by combining them with an easily measurable output, such as fluorescence. Because they are genetically encoded, BioS are cheap, fast, sustainable, portable, self-generating and highly sensitive and specific. Therefore, ...
Directed evolution of colE1 plasmid replication compatibility: a fast tractable tunable model for investigating biological orthogonality KU Leuven
Plasmids of the ColE1 family are among the most frequently used in molecular biology. They were adopted early for many biotechnology applications, and as models to study plasmid biology. Their mechanism of replication is well understood, involving specific interactions between a plasmid encoded sense-antisense gene pair (RNAI and RNAII). Due to such mechanism, two plasmids with the same origin cannot be stably maintained in cells—a process known ...
Design, synthesis and biological evaluation of pyrazolo[3,4-d] pyrimidine-based protein kinase D inhibitors KU Leuven
The multiple roles of protein kinase D (PKD) in various cancer hallmarks have been repeatedly reported. Therefore, the search for novel PKD inhibitors and their evaluation as antitumor agents has gained considerable attention. In this work, novel pyrazolo[3,4-d]pyrimidine based pan-PKD inhibitors with structural variety at position 1 were synthesized and evaluated for biological activity. Starting from 3-IN-PP1, a known PKD inhibitor with IC50 ...
Computational design targeting the androgen receptor, CCR8 receptor, and NSP15 enzyme KU Leuven
We successfully employed computational methods to discover a new AR-targeted therapy for the treatment of prostate cancer. Prostate cancer is the second most common cancer and affects millions of men each year worldwide. Targeting AR LBD dimerization could discover novel inhibitors that might overcome resistance mechanisms that occur in the current therapy. Here, we successfully developed the first-generation AR LBD dimerization inhibitors using ...
The symmetric designer protein Pizza as a scaffold for metal coordination KU Leuven
Symmetric proteins are currently of interest as they allow creation of larger assemblies and facilitate the incorporation of metal ions in the larger complexes. Recently this was demonstrated by the biomineralization of the cadmium-chloride nanocrystal via the Pizza designer protein. However, the mechanism behind this formation remained unclear. Here, we set out to investigate the mechanism driving the formation of this nanocrystal via ...
Design, synthesis and evaluation of PKD inhibitors for cancer treatment KU Leuven
Cancer is gradually becoming the leading cause of premature death in our global society. In Europe, approximately one in three develops cancer before the age of 75. With a global death rate of almost 10 million individuals per year, there is a clear need for better treatments. This thesis aimed at developing novel and potent small molecule inhibitors of Protein Kinase D (PKD), an attractive anti-cancer drug target. In first instance, novel ...