Publicaties
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Resident and bone marrow-derived mesenchymal stem cells in head and neck squamous cell carcinoma Universiteit Gent
Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells Vrije Universiteit Brussel
Abstract
PURPOSE: To investigate the spontaneous germ cell differentiation capacity of VUB hESC lines, develop a protocol for the induction of germ cell differentiation using conditioned medium from Sertoli cells (SCCM) and compare it to existing protocols.
METHODS: hESC were allowed to differentiate spontaneously or after the addition of bone morphogenetic proteins (BMPs) and/or SCCM. VASA transcripts were measured by relative ...
PURPOSE: To investigate the spontaneous germ cell differentiation capacity of VUB hESC lines, develop a protocol for the induction of germ cell differentiation using conditioned medium from Sertoli cells (SCCM) and compare it to existing protocols.
METHODS: hESC were allowed to differentiate spontaneously or after the addition of bone morphogenetic proteins (BMPs) and/or SCCM. VASA transcripts were measured by relative ...
[18F]FDG Labeling of neural stem cells for in vivo cell tracking with positron emission tomography: inhibition of tracer release by phloretin Universiteit Gent
The introduction of neural stem cells into the brain has promising therapeutic potential for the treatment of neurodegenerative diseases. To monitor the cellular replacement therapy, that is, to determine stem cell migration, survival, and differentiation, in vivo tracking methods are needed. Ideally, these tracking methods are noninvasive. Noninvasive tracking methods that have been successfully used for the visualization of blood-derived ...
Equid herpesvirus 1 (EHV1) infection of equine mesenchymal stem cells induces a pUL56-dependent downregulation of select cell surface markers Universiteit Gent
Derivation of human embryonic stem cells using a post-inner cell mass intermediate Universiteit Gent
Major depletion of SOX2(+) stem cells in the adult pituitary is not restored which does not affect hormonal cell homeostasis and remodelling KU Leuven
The pituitary gland contains SOX2-expressing stem cells. However, their functional significance remains largely unmapped. We investigated their importance by depleting SOX2+ cells through diphtheria toxin (DT)-mediated ablation. DT treatment of adult Sox2CreERT2/+;R26iDTR/+ mice (after tamoxifen-induced expression of DT receptor in SOX2+ cells) resulted in 80% obliteration of SOX2+ cells in the endocrine pituitary, coinciding with reduced ...
PDGFR alpha(+) Cells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors KU Leuven
In early mouse pre-implantation development, primitive endoderm (PrE) precursors are platelet-derived growth factor receptor alpha (PDGFRα) positive. Here, we demonstrated that cultured mouse embryonic stem cells (mESCs) express PDGFRα heterogeneously, fluctuating between a PDGFRα+ (PrE-primed) and a platelet endothelial cell adhesion molecule 1 (PECAM1)-positive state (epiblast-primed). The two surface markers can be co-detected on a third ...
Human Stem Cell Derived Liver Cells for Disease Modeling and Whole Organ Engineering KU Leuven
The vital blood system in mammals is continuously regenerated throughout life by a process called hematopoiesis. In hematopoiesis, a stem cell population called hematopoietic stem cells (HSC's) undergo differentiation and produce specialized mature blood cells including red blood cells, megakaryocytes and myeloid cells. Even a small failure in this process can result in hematological disorders such as anemia and leukemia. Transplantation of HSCs ...
Embryonic stem cells contribute to mouse chimeras in the absence of detectable cell fusion KU Leuven
Embryonic stem (ES) cells are capable of differentiating into all embryonic and adult cell types following mouse chimera production. Although injection of diploid ES cells into tetraploid blastocysts suggests that tetraploid cells have a selective disadvantage in the developing embryo, tetraploid hybrid cells, formed by cell fusion between ES cells and somatic cells, have been reported to contribute to mouse chimeras. In addition, other examples ...