Publicaties
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$K_{v}2.1$ and silent $K_{v}$ subunits underlie the delayed rectifier $K^{+}$ current in cultured small mouse DRG neurons Universiteit Antwerpen
Silent voltage-gated K+ (Kv) subunits interact with Kv2 subunits and primarily modulate the voltage dependence of inactivation of these heterotetrameric channels. Both Kv2 and silent Kv subunits are expressed in the mammalian nervous system, but little is known about their expression and function in sensory neurons. This study reports the presence of Kv2.1, Kv2.2, and silent subunit Kv6.1, Kv8.1, Kv9.1, Kv9.2, and Kv9.3 mRNA in mouse dorsal root ...
Modulation of closed−state inactivation in Kv2.1/Kv6.4 heterotetramers as mechanism for 4−AP induced potentiation Universiteit Gent Universiteit Antwerpen
The voltage-gated K+ (Kv) channel subunits Kv2.1 and Kv2.2 are expressed in almost every tissue. The diversity of Kv2 current is increased by interacting with the electrically silent Kv (KvS) subunits Kv5-Kv6 and Kv8-Kv9, into functional heterotetrameric Kv2/KvS channels. These Kv2/KvS channels possess unique biophysical properties and display a more tissue-specific expression pattern, making them more desirable pharmacological and therapeutic ...
Uncovering the epigenetic landscape of cell fate reprogramming and X chromosome reactivation KU Leuven
In this thesis, I aimed to define the dynamics and the mechanisms that orchestrate the reversal of stable gene silencing during reprogramming of somatic cells into induced pluripotent stem cells (iPSCs). I used X chromosome reactivation as a paradigm to better understand the reversal of silent chromatin. In addition, my goal was also to define the gene regulatory logic of mammalian pre- implantation development and reprogramming, as well as to ...
Auxiliary KCNE subunits modulate both homotetrameric Kv2.1 and heterotetrameric Kv2.1/Kv6.4 channels Universiteit Antwerpen
The diversity of the voltage-gated K+ (Kv) channel subfamily Kv2 is increased by interactions with auxiliary beta-subunits and by assembly with members of the modulatory so-called silent Kv subfamilies (Kv5-Kv6 and Kv8-Kv9). However, it has not yet been investigated whether these two types of modulating subunits can associate within and modify a single channel complex simultaneously. Here, we demonstrate that the transmembrane beta-subunit KCNE5 ...
Prospective, real-time monitoring of pegylated Escherichia coli and Erwinia asparaginase therapy in childhood acute lymphoblastic leukaemia and non-Hodgkin lymphoma in Belgium Universiteit Antwerpen KU Leuven Vrije Universiteit Brussel Universiteit Gent
Asparaginase (ASNase) is an important anti-leukaemic drug in the treatment of childhood acute lymphoblastic leukaemia (ALL) and non-Hodgkin lymphoma (NHL). A substantial proportion of patients develop hypersensitivity reactions with anti-ASNase neutralising antibodies, resulting in allergic reactions or silent inactivation (SI), and characterised by inactivation and rapid clearance of ASNase. We report results of a prospective, real-time ...
Recent advances in understanding the reversal of gene silencing during X chromosome reactivation. KU Leuven
Dosage compensation between XX female and XY male cells is achieved by a process known as X chromosome inactivation (XCI) in mammals. XCI is initiated early during development in female cells and is subsequently stably maintained in most somatic cells. Despite its stability, the robust transcriptional silencing of XCI is reversible, in the embryo and also in a number of reprogramming settings. Although XCI has been intensively studied, the ...
Development and characterization of a linear human chromosomal vector to study increased gene dosage in mice KU Leuven
Scientific summaryThe main focus of the human genome lab is the study of X-chromosome Linked Intellectual Disability (XLID). One aspect is to screen XLID-patients for copy-number variations (CNVs) using an in house-developed X-BAC-array, that covers the entire X-chromosome with a resolution of 80 kb, and more recently, an X-oligo-array with a much higher resolution. In addition, our research focuses on the functional characterization of the ...
Insights from zebrafish deficiency models to understand the impact of local thyroid hormone regulator action on early development KU Leuven
Thyroid hormones (THs) stimulate and coordinate a wide range of processes to ensure normal development, mainly by binding of the most active TH 3,5,3'-triiodothyronine (T3) to nuclear receptors resulting in changes in gene transcription. Local TH action is monitored at three distinct levels by different types of regulators: transmembrane transporters (TH influx and efflux), deiodinases (TH activation and inactivation) and nuclear receptors (TH ...
Acute Effect of Noradrenergic Modulation on Motor Output Adjustment in Men Vrije Universiteit Brussel
PURPOSE: To determine the role of noradrenergic modulation in the control of motor output, we compared the acute effect of reboxetine (REB), a noradrenaline reuptake inhibitor, to a placebo (PLA) on knee extensors motor performance and cortical and spinal excitability.
METHODS: Eleven young men took part in two randomized experiments during which they received either 8 mg of REB or a PLA. The torque produced during a maximal voluntary ...