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Direct reprogramming of somatic cells into induced hepatocytes Vrije Universiteit Brussel

Matthias Rombaut, Joost Boeckmans, Robim Rodrigues, Leo A van Grunsven, Tamara Vanhaecke, Joery De Kock

There is an unmet need for functional primary human hepatocytes to support the pharmaceutical and (bio)medical demand. The unique discovery, a decade ago, that somatic cells can be drawn out of their apparent biological lockdown to reacquire a pluripotent state has revealed a completely new avenue of possibilities for generating surrogate human hepatocytes. Since then, the number of papers reporting the direct conversion of somatic cells into ...

Reprogramming of human exocrine pancreas cells to beta cells Vrije Universiteit Brussel Universiteit Gent

Willem Staels, Yves Heremans, Henry Heimberg

One of the key promises of regenerative medicine is providing a cure for diabetes. Cell-based therapies are proving their safety and efficiency, but donor beta cell shortages and immunological issues remain major hurdles. Reprogramming of human pancreatic exocrine cells towards beta cells would offer a major advantage by providing an abundant and autologous source of beta cells. Over the past decade our understanding of transdifferentiation ...

Transient cytokine treatment induces acinar cell reprogramming and regenerates functional beta cell mass in diabetic mice Vrije Universiteit Brussel

Luc Baeyens, Marie Lemper, Gunter Leuckx, Sofie De Groef, Paola Bonfanti, Geert Stange, Ruth Shemer, Christoffer Nord, David Scheel, Fong Pan, et al.
The regenerative potential of terminally differentiated cells in mammals remains poorly understood. Unveiling signaling pathways responsible for activation of cellular plasticity might allow major advances in regenerative medicine. Pancreas non-beta cells, that remain unaffected by prior diabetogenic attacks, could provide an alternative therapy for diabetes if reprogrammed to functional beta-like cells. The present study provides proof of ...

In vitro reprogramming of pancreatic alpha cells towards a beta cell phenotype following ectopic HNF4α expression Vrije Universiteit Brussel

Caroline B Sangan, Ramiro Jover, Henry Heimberg, David Tosh

There is currently a shortage of organ donors available for pancreatic beta cell transplantation into diabetic patients. An alternative source of beta cells is pre-existing pancreatic cells. While we know that beta cells can arise directly from alpha cells during pancreatic regeneration we do not understand the molecular basis for the switch in phenotype. The aim of the present study was to investigate if hepatocyte nuclear factor 4 alpha ...

Suppression of epithelial to mesenchymal transitioning (EMT) enhances ex vivo reprogramming of human exocrine pancreatic tissue towards functional insulin producing β-like cells Vrije Universiteit Brussel

Maria Joao Lima, Kenneth Muir, Hilary Docherty, Robert Drummond, Neil Mcgowan, Shareen Forbes, I Houbracken, James Ross, Philippe Ravassard, John Casey, et al.
Due to the lack of tissue available for islet transplantation, new sources of beta-cells have been sought for the treatment of type 1 diabetes. The aim of this study was to determine whether the human exocrid fraction from the islet isolation procedure could be reprogrammed to provide additional islet tissue for transplantation. The exocrine enriched cells rapidly dedifferentiated in culture and grew as a mesenchymal monolayer. Genetic lineage ...

Microenvironmental reprogramming of thymic epithelial cells to skin multipotent stem cells. Vrije Universiteit Brussel

Paola Bonfanti, S Claudinot, Aw Amici, A Farley, Cc Blackburn, Yan Barrandon
The thymus develops from the third pharyngeal pouch of the anterior gut and provides the necessary environment for thymopoiesis (the process by which thymocytes differentiate into mature T lymphocytes) and the establishment and maintenance of self-tolerance. It contains thymic epithelial cells (TECs) that form a complex three-dimensional network organized in cortical and medullary compartments, the organization of which is notably different from ...

Gamifying programming education in K‐12: A review of programming curricula in seven countries and programming games Vrije Universiteit Brussel

Renny Lindberg, Teemu Laine, Lassi Haaranen
An increasing number of countries have recently included programming education in their curricula. Similarly, utilizing programming concepts in gameplay has become popular in the videogame industry. Although many games have been developed for learning to program, their variety and their correspondence to national curricula remain an uncharted territory. Consequently, this paper has three objectives. Firstly, an investigation on the guidelines on ...