Publicaties
The development and validation of a novel Nanobody-Based competitive ELISA for the detection of foot and mouth disease 3ABC antibodies in cattle Vrije Universiteit Brussel
Effective management of foot and mouth disease (FMD) requires diagnostic tests to distinguish between infected and vaccinated animals (DIVA). To address this need, several enzyme-linked immunosorbent assay (ELISA) platforms have been developed, however, these tests vary in their sensitivity and specificity and are very expensive for developing countries. Camelid-derived single-domain antibodies fragments so-called Nanobodies, have ...
Combining in-situ proteolysis and microseed matrix screening to promote crystallization of PrPc-nanobody complexes Vrije Universiteit Brussel
Prion proteins (PrPs) are difficult to crystallize, probably due to their inherent flexibility. Several PrPs structures have been solved by nuclear magnetic resonance (NMR) techniques; however, only three structures were solved by X-ray crystallography. Here we combined in-situ proteolysis with automated microseed matrix screening (MMS) to crystallize two different PrP(C)-nanobody (Nb) complexes. Nanobodies are single-domain antibodies ...
Imaging and radioimmunotherapy of multiple myeloma with anti-idiotypic Nanobodies Vrije Universiteit Brussel
(CR). Therefore, ...
Delayed PLAG1-induced tumorigenesis in a conditional fur knockout mouse model: an incentive for a search for furin inhibitors KU Leuven
Stratifying fascin and cortactin function in invadopodium formation using inhibitory nanobodies and targeted subcellular delocalization Vrije Universiteit Brussel
A non-internalised CD38-binding radiolabelled single-domain antibody fragment to monitor and treat multiple myeloma Vrije Universiteit Brussel
BACKGROUND: Antibody-based therapies targeting CD38 are currently used as single agents as well as in combination regimens for multiple myeloma, a malignant plasma cell disorder. In this study, we aimed to develop anti-CD38 single-domain antibodies (sdAbs) that can be used to trace CD38+ tumour cells and subsequently used for targeted radionuclide therapy. SdAbs are derived from Camelidae heavy-chain antibodies and have emerged as promising ...
Improved detection of molecular markers of atherosclerotic plaques using sub-millimeter PET imaging Universiteit Gent KU Leuven Vrije Universiteit Brussel
Same-day imaging using small proteins Vrije Universiteit Brussel
Imaging of expression of therapeutic targets may enable stratification of patients for targeted treatments. The use of small radiolabeled probes based on the heavy-chain variable region of heavy-chain–only immunoglobulins or nonimmunoglobulin scaffolds permits rapid localization of radiotracers in tumors and rapid clearance from normal tissues. This makes high-contrast imaging possible on the day of injection. This mini review focuses on ...
Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes Vrije Universiteit Brussel
Bloom's syndrome helicase (BLM) is a member of the RecQ family of DNA helicases, which play key roles in the maintenance of genome integrity in all organism groups. We describe crystal structures of the BLM helicase domain in complex with DNA and with an antibody fragment, as well as SAXS and domain association studies in solution. We show an unexpected nucleotide-dependent interaction of the core helicase domain with the conserved, poorly ...