Publicaties
Gekozen filters:
Gekozen filters:
Combining multiplexed gel capillary electrophoresis with liquid chromatography for offline comprehensive analysis of complex oligonucleotide samples Universiteit Gent
Enantioseparation of pharmaceutical compounds by multiplexed capillary electrophoresis using highly sulphated alpha-, beta- and gamma-cyclodextrins Universiteit Gent
A multiplexed capillary electrophoresis (CE) system equipped with 96 channels was evaluated for high-throughput screening of enantiomers of solutes of pharmaceutical interest. Using highly (DS similar to 12) sulphated alpha-, beta- and gamma-cyclodextrins under acidic conditions (pH 2.5) only 48 channels could be used because of the high conductivity of the chiral selectors. method transfer from a single channel to a 48 channel CE system is ...
Multiplexed protein detection using an affinity aptamer amplification assay KU Leuven
Affinity probe capillary electrophoresis (APCE) is potentially one of the most versatile technologies for protein diagnostics, offering an excellent balance between robustness, analysis speed and sensitivity. Combining the immunosensing and separating strength of capillary electrophoresis with the signal enhancement power of nucleic acid amplification, aptamers can further push the analytical limits of APCE to offer ultrasensitive, multiplexed ...
Hyphenating chromatographic, electrophoretic and on-line immobilized enzymatic strategies for improved oligonucleotide analysis Universiteit Gent
Interlaboratory diagnostic validation of conformation-sensitive capillary electrophoresis for mutation scanning Vrije Universiteit Brussel KU Leuven
BACKGROUND: Indirect alternatives to sequencing as a method for mutation scanning are of interest to diagnostic laboratories because they have the potential for considerable savings in both time and costs. Ideally, such methods should be simple, rapid, and highly sensitive, and they should be validated formally to a very high standard. Currently, most reported methods lack one or more of these characteristics. We describe the optimization and ...
Interlaboratory Diagnostic Validation of Conformation-Sensitive Capillary Electrophoresis for Mutation Scanning KU Leuven
BACKGROUND: Indirect alternatives to sequencing as a method for mutation scanning are of interest to diagnostic laboratories because they have the potential for considerable savings in both time and costs. Ideally, such methods should be simple, rapid, and highly sensitive, and they should be validated formally to a very high standard. Currently, most reported methods lack one or more of these characteristics. We describe the optimization and ...
Pulmonary vascular endothelialitis, thrombosis, and angiogenesis in Covid-19 Universiteit Antwerpen KU Leuven
BackgroundProgressive respiratory failure is the primary cause of death in the coronavirus disease 2019 (Covid-19) pandemic. Despite widespread interest in the pathophysiology of the disease, relatively little is known about the associated morphologic and molecular changes in the peripheral lung of patients who die from Covid-19. MethodsWe examined 7 lungs obtained during autopsy from patients who died from Covid-19 and compared them with 7 ...
Development and multiplexing of microsatellite markers using pyrosequencing in a tetraploid plant, Vaccinium uliginosum (Ericaceae) Plantentuin Meise / Agentschap Plantentuin Meise
Objective: Background and aims - We developed a new set of microsatellite primers in the tetraploid perennialshrub, Vaccinium uliginosum L., to investigate genetic diversity and population genetic structure.Methods - Using pyrosequencing, we identifi{ligature}ed and designed primers for PCR amplification ofmicrosatellite loci in V. uliginosum. The primers were first screened for amplification and polymorphismby PCR and agarose gel ...