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Disruption of SF3B1 results in deregulated expression and splicing of key genes and pathways in myelodysplastic syndrome hematopoietic stem and progenitor cells KU Leuven

H Dolatshad, A Pellagatti, M Fernandez-Mercado, BH Yip, L Malcovati, M Attwood, B Przychodzen, N Sahgal, AA Kanapin, H Lockstone, et al.
The splicing factor SF3B1 is the most commonly mutated gene in the myelodysplastic syndromes (MDS), particularly in patients with refractory anemia with ring sideroblasts (RARS). We investigated the functional effects of SF3B1 disruption in myeloid cell lines: SF3B1 knockdown resulted in growth inhibition, cell cycle arrest and impaired erythroid differentiation, and deregulation of many genes and pathways, including cell cycle regulation and ...

Next generation of ALDH substrates and their potential to study maturational lineage biology in stem and progenitor cells Vrije Universiteit Brussel

Laurent Dolle, Leonardus van Grunsven

High aldehyde dehydrogenase (ALDH) activity is a feature of stem cells from normal and cancerous tissues and a reliable universal marker used to isolate them. There are numerous ALDH isoforms with preferred substrate specificity variably expressed depending on tissue, cell type, and organelle and cell status. On the other hand, a given substrate may be metabolized by several enzyme isoforms. Currently ALDH activity is evidenced by using ...

18F-FDG Labeling of Mesenchymal Stem Cells and Multipotent Adult Progenitor Cells for PET Imaging: Effects on Ultrastructure and Differentiation Capacity KU Leuven

Esther Wolfs, Tom Struys, Tineke Notelaers, Scott Roberts, Abishek Sohni, Guy Bormans, Koenraad Van Laere, Frank Luyten, Olivier Gheysens, Ivo Lambrichts, et al.
Because of their extended differentiation capacity, stem cells have gained great interest in the field of regenerative medicine. For the development of therapeutic strategies, more knowledge on the in vivo fate of these cells has to be acquired. Therefore, stem cells can be labeled with radioactive tracer molecules such as (18)F-FDG, a positron-emitting glucose analog that is taken up and metabolically trapped by the cells. The aim of this study ...

Stem and progenitor cells for liver repopulation: can we standardise the process from bench to bedside? KU Leuven

Pau Sancho Bru, M Najimi, M Caruso, Karen Pauwelyn, T Cantz, S Forbes, Tania Roskams, M Ott, U Gehling, E Sokal, et al.
There has been recent progress in the isolation and characterisation of stem/progenitor cells that may differentiate towards the hepatic lineage. This has raised expectations that therapy of genetic or acquired liver disease might be possible by transplanting stem/progenitor cells or their liver-committed progeny. However, it is currently impossible to determine from the many documented studies which of the stem/progenitor cell populations are ...

Genetic and Epigenetic Modification of Rat Liver Progenitor Cells via HNF4α Transduction and 5’ Azacytidine Treatment: An Integrated miRNA and mRNA Expression Profile Analysis Vrije Universiteit Brussel

Jennifer Bolleyn, Matthias Rombaut, N. Nair, Steven Branson, Anja Heymans, Anja Heymans, Marinee Chuah, Thierry Vandendriessche, Vera Rogiers, Joery De Kock, et al.

Neonatal liver-derived rat epithelial cells (rLEC) from biliary origin are liver progenitor cells that acquire a hepatocyte-like phenotype upon sequential exposure to hepatogenic growth factors and cytokines. Undifferentiated rLEC express several liver-enriched transcription factors, including the hepatocyte nuclear factors (HNF) 3β and HNF6, but not the hepatic master regulator HNF4α. In this study, we first investigated the impact of the ...

Role of stem and progenitor cells in postmyocardial infarction patients KU Leuven

X Liu, Dieter Dauwe, Abdulsamie PATEL, Stefan Janssens
Despite state-of-the-art therapy, clinical outcome remains poor in myocardial infarction (MI) patients with reduced left ventricular (LV) function. Stem cell-mediated repair of the damaged heart is a promising new development in cardiovascular medicine. Embryonic stem cells and adult progenitor cells have been extensively studied for their capacity to improve LV function recovery in preclinical MI models but underlying mechanisms remain ...

Adult Stem and Progenitor Cells KU Leuven

Martine Geraerts, Catherine Verfaillie
The discovery of adult stem cells in most adult tissues is the basis of a number of clinical studies that are carried out, with therapeutic use of hematopoietic stem cells as a prime example. Intense scientific debate is still ongoing as to whether adult stem cells may have a greater plasticity than previously thought. Although cells with some features of embryonic stem cells that, among others, express Oct4, Nanog and SSEA1 are isolated from ...