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Kanamycin susceptibility testing of Mycobacterium tuberculosis using mycobacterium growth indicator tube and a colorimetric method Instituut voor Tropische Geneeskunde
Single-dose kanamycin therapy of gonococcal ophthalmia neonatorum Instituut voor Tropische Geneeskunde
Thin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolates Instituut voor Tropische Geneeskunde
High level of cross-resistance between kanamycin, amikacin, and capreomycin among **Mycobacterium tuberculosis** isolates from Georgia and a close relation with mutations in the rrs gene Universiteit Antwerpen
The aminoglycosides kanamycin and amikacin and the macrocyclic peptide capreomycin are key drugs for the treatment of multidrug-resistant tuberculosis (MDR-TB). The increasing rates of resistance to these drugs and the possible cross-resistance between them are concerns for MDR-TB therapy. Mutations in the 16S rRNA gene (rrs) have been associated with resistance to each of the drugs, and mutations of the tlyA gene, which encodes a putative rRNA ...
Capacitively coupled contactless conductivity detection as an alternative detection mode in CE for the analysis of kanamycin sulphate and its related substances KU Leuven
A method was developed to determine simultaneously kanamycin, its related substances and sulphate in kanamycin sulphate using capacitively coupled contactless conductivity detection. Kanamycin is an aminoglycoside antibiotic that lacks a strong UV-absorbing chromophore. Due to its physicochemical properties, CE in combination with capacitively coupled contactless conductivity detection was chosen. The separation method uses a BGE composed of 40 ...
Assay of Kanamycin A by HPLC with Direct UV Detection KU Leuven
The development of a simple reversed phase ion pair liquid chromatographic method for the assay of kanamycin A has been described. Because of the lack of a UV chromophore in the structure of kanamycin A, borate complexation was used to allow direct UV detection at 205 nm. Three columns were evaluated in this study: Zorbax Extend C18 (4.6 mm × 250 mm; 5 μm), XBridge C18 (4.6 mm × 250 mm; 5 μm) and apHera C18 (4.6 mm × 250 mm; 5 μm). The mobile ...
Improved liquid chromatographic method with pulsed electrochemical detection for the analysis of kanamycin KU Leuven
This work describes the separation of the main component kanamycin A from its related substances using an improved liquid chromatographic method with pulsed electrochemical detection (LC-PED). Two methods, one using volatile ion pairing agents and the other using non-volatile ones were developed. Using volatile additives, the total run time was rather long with no possibility of developing gradient elution. The non-volatile method was found to ...
Thin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolates Universiteit Gent
BACKGROUND: In low-income countries there is a great need for economical methods for testing the susceptibility of Mycobacterium tuberculosis to antibiotics.OBJECTIVE: To evaluate the thin-layer agar (TLA) for rapid detection of resistance to rifampicin (RMP), ofloxacin (OFX) and kanamycin (KM) in M. tuberculosis clinical isolates and to determine the sensitivity, specificity and time to positivity compared to the gold standard method.METHODS: ...
Target of rapamycin (TOR) determines appendage size during pupa formation of the red flour beetle Tribolium castaneum Universiteit Gent
The adult body size is species-specific and controlled by complex interactions between hormones and the IIS/TOR pathway. To analyze the role of target of rapamycin (TOR) in the growth and development of the insect, expression levels of TOR were silenced in the model and pest insect red flour beetle, Tribolium castaneum. Injection of dsRNA into the last larval instar decreased pupal mass and size, while the amount of food intake by the larvae was ...